LOCUS KT224358 6100 bp RNA linear VRL 28-MAR-2016 DEFINITION Astrovirus MLB2 isolate MLB2/human/Geneva/2014, complete genome. ACCESSION KT224358 VERSION KT224358.1 KEYWORDS . SOURCE Astrovirus MLB2 ORGANISM Astrovirus MLB2 Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae; unclassified Astroviridae. REFERENCE 1 (bases 1 to 6100) AUTHORS Cordey,S., Vu,D.-L., Schibler,M., L'Huillier,A.G., Brito,F., Docquier,M., Posfay-Barbe,K.M., Petty,T.J., Turin,L., Zdobnov,E.M. and Kaiser,L. TITLE Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection JOURNAL Emerging Infect. Dis. 22 (5), 846-853 (2016) PUBMED 27088842 REFERENCE 2 (bases 1 to 6100) AUTHORS Cordey,S., Vu,D.-L., Schibler,M., L'Huillier,A.G., Brito,F., Docquier,M., Posfay-Barbe,K., Petty,T., Tapparel,C., Turin,L., Preynat-Seauve,O., Zdobnov,E.M. and Kaiser,L. TITLE Direct Submission JOURNAL Submitted (29-JUN-2015) Laboratory of Virology, Infectious Diseases Service, University Hospitals of Geneva, Rue Gabrielle-Perret-Gentil 4, Geneva 1211, Switzerland COMMENT ##Assembly-Data-START## Assembly Method :: Sparse Assembler v. june 2105 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6100 /mol_type="genomic RNA" /country="Switzerland" /collection_date="Sep-2014" /host="Homo sapiens" /db_xref="taxon:683172" /isolate="MLB2/human/Geneva/2014" /organism="Astrovirus MLB2" CDS 3..2366 /codon_start=1 /protein_id="AMR45106.1" /translation="MAVPFHIGVADKVLTLGSSRSRMWLNELSPAVQNQLRDIFPYEV PLNTMIVPFSRYSMGTGVTLAVQGITTPGVYSTYVLHGESWLEVDEERVEPACAFVGA LAVDRTKLAERNRELQNDNASIIVENQLLRHEVERLRPPATRPVRFSNFALFVLFFFL FFIIGTKAENWQDQTKDYVIDFLRDVKYEFISNLDIYYSVLLNLIRWEVISCVIAIVS FFKLKRPCVAIIGLFLATISRYNYSALSIVPYLDIYSTAFLYVLMVVYFLVPHVAIVT SVVCAMLFTIVSMVCPHHQFIMRVRGDWLVVLVTIATYICDSMGIHTTAIAIMAAVAR IYMCMPATTASVVEIKDNTGKVVQKTTLMPNWIGTMYQSARGMFQRVRTGVALFVRVN PNCLCHIKVDGCSGTGFRLGNDIVTAAHVIGNATQVEVVYNNYVAQAKVRHIPDKDIA YLVLPEGLKSMPVLKLARTPSYEQVTIIALEGNCMLVSTTEGVCHGETISYACATRDG MSGAPVLDVNGNVLGVHQTNTGYTGGATVIRATDAAPYVDENAALKKEIEELKRIIKE NAMQQRSVQDNEVVGIVRAAVQREIGILRDELNSCYQKKKGKNKKGRGARHRMLRRGQ KFLTEEEYQELLDKGLTREQLLDAIEEIVRSRIGFPDWSDPEYSSEEDDAAITYWWEQ VKEDNYKDGIPQFDHIIPKYSPSERTFSEQERKDVARELDALQSVVDKVNKDEWDEKK KQITDELNHALFVVDKALAQHGYILFEQRMRPKNGKRGPRRRTPNTT" /product="putative serine protease" CDS <2303..3838 /codon_start=1 /protein_id="AMR45105.1" /translation="TKDEAKKRKEGSSQKDPKHYLDSWIGMIMPCARWLVPERYPLYG GVPLDRPVFDYKVVNDDLLGLLPPPPNQEFDVFGPTVWDEIAYKKSFEKFEYAEYSDF LNVYIECVAFADSRFLEHFNFLSESGFTHILGTDKNMESTPAYPKMRWWLTEEDYLDE HGWEYYEKEFDNIASGQNFDVCWYLFLKKEILRQSKIDEGDIRQIVCADPIYSRIGAC FEQHQNRMMKDYTEKSSGQCGWSPFFGGFEQRMDRLEKKGNKFYVELDWTRFDGTIPP TLLMHIKKLRFSLMGEVAHKYERVYKWYCRNLVNRFVILPSGEVTLQNRGNPSGQIST TMDNNMINYWLQAFEFKYLGLPEDEWIHFDTIVYGDDRLSTYRTLPADYTSKVVAMYK DVFGMWVKPEKVKVSDTLIGLSFCGFTNTKKGPAPSEPYKLMASLLKPVSKLPDIVAL HGKLLCFQLLMANNADHPFYGYVEQCLHYTHRALSDANLPRRFTRRQLEYIWRGGPNG DYG" /product="putative RdRp" /note="translated by ribosomal frameshift" CDS 3831..6068 /codon_start=1 /protein_id="AMR45107.1" /translation="MANANKGVTINVGNVKRKQRAASNQRIRSAGPGPKRASKLRKRW HFPNRNRRRRTHLNTTGPKPAVSQTITATLGTVGSNLSDVVETECAVFLNPVVAKDSG ASATFGPLQSLGAQYALWRLKWLEVRLQPLVGSSAVSGTVVRVSLNMTTGPTLNSWSG LGARFHRDVRVGSGLVWKITQRHVSGPCETWWKTNTAEDPSLSLGPALEVHTIGKTMS TYKNETFTSGLFLLEITGHWEFANYAASPALATLTQHKDDSETTKTLAISAEGANEPV EMLIPVGKWNMKEQYGGNGTLGEAIYQVVDTSVTVVTDLFPPPFSWLGKASWWFIRKV IGKSKTYTLTGTNQQITADVYRVYTSASAARDDKPAISSTAHQAKNLTGSVVITQIST DNILNETTYTGPRSVLPDQPIGPSSYPMTPDSLVLMAGYFRAPEINENFGRYMPYVFQ QRSEVIKFVSGTHHTMVVGFWTVDRTMWLDKHFNHFTNEPDGVFGEVGNILINGDNVA KVITMSGSSATSERGATLMLCKTTKPVQTFSVNADVFIPAYRVIDGQSSKDIKIQINS WEDNKQLQFTSMPTGTYFMVLTMGGGTFVVKNYVVYNERINNDLQLPAFWGKYLSQAF GLSWSSPTYACVTWEPIYSNEPLPHRTIEWDESSDEDEEPDEFFDEDPIATLHAVDAE RKIRRAIFNALMEEGVDAGRSAEYAARACPTEIQKRRRGLYESLLSDGLSPETSYSEA CLLDK" /product="putative capsid protein" ORIGIN 1 ggatggctgt gcctttccac attggtgttg cagataaggt tttgacatta ggttcctcta 61 ggtctcgtat gtggcttaat gaattgtcac cagctgtaca gaaccagctt cgagacattt 121 tcccttatga ggtacctcta aacacaatga ttgtgccttt ttcccgttat tctatgggga 181 ctggagttac gctagctgtc caaggtatca ccacaccagg tgtatatagt acctatgttt 241 tacatggtga atcctggttg gaggttgacg aagaacgtgt tgaacctgcc tgtgcatttg 301 ttggtgcact tgcagtggac agaacaaagc tagcagagcg taaccgtgaa ttgcagaatg 361 ataatgcatc aatcattgtg gaaaaccaac ttctgcgaca tgaagtggag aggctcaggc 421 cacctgctac tcgaccagtg cgttttagta atttcgcact atttgtcctc ttctttttcc 481 ttttctttat tattggaact aaggcagaga attggcaaga tcaaactaaa gattatgtaa 541 ttgacttttt aagggatgta aagtatgaat ttatatctaa tttagatata tattactctg 601 tcttattaaa tcttattagg tgggaagtca tttcatgtgt tattgccata gtttccttct 661 ttaaattgaa aagaccatgt gttgccataa ttggtttgtt tttggcgact atttcaaggt 721 ataattattc agcattgtct attgtgccat atttagacat ttacagtact gcttttttat 781 atgtccttat ggttgtctac tttttggtgc cacatgttgc tatagttaca tcagttgttt 841 gtgctatgct attcactatt gttagtatgg tatgccctca tcaccagttt atcatgcgtg 901 ttcgtggtga ctggttggtt gttttagtca caattgccac ttatatatgt gattccatgg 961 gcatacatac cacagccatt gctataatgg ctgctgttgc acgtatatat atgtgtatgc 1021 cggctaccac tgcatctgtt gttgagataa aggataacac tggtaaggtt gttcaaaaaa 1081 ccacacttat gccaaattgg ataggaacaa tgtatcagag tgcacgtggc atgttccagc 1141 gtgtacgtac tggtgtggca ttattcgtta gggttaatcc taactgttta tgccacatca 1201 aggttgatgg ttgcagtggt actggtttta gattaggaaa tgatatagtg acagcagcac 1261 atgtcattgg taatgccaca caggttgaag ttgtgtacaa caattatgtg gcccaggcta 1321 aagttagaca tatacctgat aaggacattg cttatttagt acttccagag ggattaaagt 1381 caatgccagt gttgaagtta gcacgtacac catcatatga gcaagtgaca attattgctt 1441 tagaaggtaa ttgcatgctt gtatcaacta ccgaaggtgt gtgccatggt gaaacaatat 1501 catatgcatg tgccacacgt gatggtatga gtggagcacc tgttttagat gttaatggta 1561 atgttcttgg tgttcatcaa actaacactg gttatactgg tggggccact gtcatacgtg 1621 cgacagatgc tgccccatat gttgatgaaa atgctgcctt gaaaaaagaa attgaggaat 1681 taaaaagaat catcaaagaa aatgcaatgc aacagagatc tgttcaagat aatgaggttg 1741 ttgggattgt acgtgctgca gtccaacgcg aaattggtat ccttcgcgat gagctaaact 1801 cttgttatca gaagaagaaa ggaaaaaaca aaaaaggccg tggcgcacgt catagaatgt 1861 tgcgtcgtgg tcaaaaattt ttgactgaag aagaatatca ggaattattg gataaaggcc 1921 taaccagaga acaattgctt gatgctatag aagagatagt taggtctagg ataggttttc 1981 cagactggtc tgatcctgag tattcatcag aggaggatga tgctgccatc acatactggt 2041 gggagcaagt aaaagaggac aactacaaag atggaatacc tcaatttgac cacataattc 2101 caaaatattc accttctgaa cgtacctttt ctgagcagga gcgtaaggac gttgcgaggg 2161 aattagatgc attgcaatct gttgtagata aagtcaataa ggatgagtgg gatgaaaaga 2221 aaaaacagat tactgatgaa ttgaatcatg cactatttgt agtagataaa gcccttgccc 2281 aacatgggta tatattgttt gaacaaagga tgaggccaaa aaacggaaag aggggtcctc 2341 gcagaaggac cccaaacact acttagactc gtggattggt atgataatgc catgcgctcg 2401 atggctggtt ccagagcgct atccactgta tggtggtgta ccgttagata ggcctgtgtt 2461 tgattataag gttgttaatg atgatttgct tgggctttta cctcccccac ctaatcaaga 2521 atttgatgtt tttggtccaa ctgtttggga tgaaattgca tataagaaat cctttgaaaa 2581 gtttgaatat gctgaatata gtgatttttt aaatgtatat attgaatgtg tagcttttgc 2641 agatagtagg tttttagaac actttaattt tttgtctgaa tctggattta cacatatact 2701 tggaacagat aaaaatatgg aatccacacc tgcatatcca aaaatgagat ggtggttaac 2761 agaagaggat tatcttgatg agcatggttg ggagtactat gaaaaagaat ttgacaatat 2821 tgcatctggc cagaattttg atgtctgttg gtatcttttc ttaaagaaag aaattttgag 2881 gcaatcaaag attgacgaag gtgatatcag acaaattgta tgtgcagatc ctatatactc 2941 tcgtatagga gcttgctttg aacagcacca aaatagaatg atgaaagact acactgaaaa 3001 atcctctggc cagtgtggat ggtctccctt ctttggaggc tttgaacaac gtatggatag 3061 gcttgaaaag aaaggcaata aattctatgt tgagcttgac tggacacgat ttgatggtac 3121 gataccacct actcttttga tgcatatcaa aaaattacgg ttctcgctta tgggtgaggt 3181 tgcccataaa tatgaaagag tgtataaatg gtattgtagg aacctagtta ataggttcgt 3241 tatactccct tctggtgagg tcactctgca gaaccgtggt aatccatccg gtcaaatatc 3301 aactacaatg gacaataata tgattaatta ttggttgcaa gcctttgagt tcaaatattt 3361 gggcctacct gaagatgaat ggatacattt tgacactatt gtgtatggtg atgataggct 3421 ctctacctat cgcactttac cagctgatta cacctccaaa gttgtagcca tgtataaaga 3481 tgtctttgga atgtgggtaa agccagagaa agttaaggtc tcagacactc tcattggtct 3541 atcattctgt ggattcacaa atactaagaa aggacctgca cctagtgaac catataagtt 3601 aatggcttca ctacttaaac ctgtgagcaa actacctgac attgtagccc tgcatgggaa 3661 acttttgtgc ttccagttgt tgatggcaaa taacgctgat cacccatttt atgggtatgt 3721 tgagcaatgc ctgcattata cacaccgtgc tcttagtgat gcgaacttac caaggcgctt 3781 cactcggaga caattggagt acatttggag gggtggacca aatggagatt atggctaatg 3841 ccaataaggg tgtgacaatt aatgtaggaa atgtcaaacg taagcagcgt gctgccagta 3901 accagcgaat tagatctgct gggccaggtc ctaagcgagc atccaagctt aggaaacgtt 3961 ggcattttcc taatcggaat cgccgtcgta ggacgcatct taatacaact gggcctaagc 4021 ctgcggtgtc acagacgatc acagcaacac ttggcaccgt tggatcaaat ttgagtgatg 4081 ttgttgagac agaatgtgca gtgttcctta atcctgttgt ggccaaagat tctggggcta 4141 gtgccacctt cgggccctta caatcattag gggcccaata tgcattatgg agacttaagt 4201 ggctggaggt tcgcctccag ccattagttg gtagttctgc tgttagtggc actgtagtta 4261 gagttagtct taacatgaca actggcccta cattgaattc atggtcgggt cttggtgcgc 4321 gatttcatcg agatgtgcgt gtcggatctg gcctagtgtg gaaaattaca cagagacatg 4381 tttcaggccc ttgtgaaact tggtggaaaa caaatacagc agaagatccc tcactatcac 4441 ttggtcctgc attggaagtc cacacaattg ggaagacaat gagtacttac aaaaatgaaa 4501 cttttactag tggacttttt ctcttggaga ttacaggaca ttgggagttt gctaattatg 4561 ctgcatcacc agcattggca actttaaccc aacataaaga tgatagtgag actactaaga 4621 ctcttgctat aagtgcggag ggagcaaatg aaccagttga aatgttaatt cctgttggca 4681 agtggaatat gaaagagcag tatggtggaa atggtacact tggtgaagca atttaccaag 4741 ttgttgacac tagtgtgact gttgttacag atctttttcc accaccattt tcatggcttg 4801 ggaaagcatc atggtggttt atcaggaaag ttataggtaa aagcaaaacc tatactctaa 4861 caggaacaaa tcaacaaata acagctgatg tttacagagt ttacacatct gcttccgcag 4921 caagagatga taaaccagca atttctagta ctgcccacca ggcaaaaaac ctcacagggt 4981 cagttgtgat tacccagata tctactgata atattttgaa tgagacaaca tatactggac 5041 ccagatctgt gctaccagac caacccattg gaccatcatc atatccaatg accccagact 5101 cacttgtttt gatggctggt tatttcaggg cccctgaaat aaatgagaat tttggcagat 5161 acatgccata tgttttccag cagcgaagtg aggtgatcaa atttgttagt ggtacacacc 5221 atactatggt tgttggtttt tggactgttg atagaaccat gtggttggat aaacacttta 5281 accactttac taatgaacca gatggtgtgt ttggtgaggt tgggaacatc ctcatcaatg 5341 gtgataatgt tgctaaagtt atcaccatga gtggttcatc tgcaacatct gagaggggag 5401 ccactttaat gctttgtaaa accactaaac cagtacaaac ctttagtgtt aatgctgatg 5461 ttttcatacc agcatacagg gtcattgatg gtcaaagtag taaggatatc aaaatccaga 5521 ttaattcatg ggaagataac aaacagctcc agtttacatc aatgcctact ggcacttatt 5581 ttatggtcct cactatgggt ggtggcacat ttgtagttaa gaactatgtt gtttataatg 5641 aaagaataaa caatgacctt caactcccag ctttttgggg gaaatattta tctcaagctt 5701 ttggcttgag ttggagctca ccaacatatg cctgtgtcac atgggaaccc atttacagta 5761 atgaaccact accacatcgt accatagaat gggacgagag tagtgacgaa gatgaagaac 5821 cagatgaatt ttttgatgag gaccccatag ctacactaca tgcagttgat gcagagagaa 5881 aaatccggcg tgcaatattc aatgctctca tggaagaggg tgttgatgct ggccgctctg 5941 cagaatatgc agcaagagca tgccctactg aaattcagaa gaggaggaga ggactctatg 6001 aaagcttgct gtccgacggc ttatcacctg agacgtcgta cagcgaagca tgtcttttag 6061 acaaataaaa ttttatcttt ttttttctaa tcaggtagat //