LOCUS GQ502193 6531 bp RNA linear VRL 25-SEP-2012 DEFINITION Astrovirus VA2 isolate VA2/human/Stl/WD0680/2009, complete genome. ACCESSION GQ502193 VERSION GQ502193.2 KEYWORDS . SOURCE Astrovirus VA2 ORGANISM Astrovirus VA2 Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae; Mamastrovirus; unclassified Mamastrovirus. REFERENCE 1 (bases 1 to 6531) AUTHORS Finkbeiner,S.R., Holtz,L.R., Jiang,Y., Rajendran,P., Franz,C.J., Zhao,G., Kang,G. and Wang,D. TITLE Human stool contains a previously unrecognized diversity of novel astroviruses JOURNAL Virol. J. 6, 161 (2009) PUBMED 19814825 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 6531) AUTHORS Finkbeiner,S.R., Holtz,L.R., Jiang,Y., Rajendran,P., Franz,C.J., Zhao,G., Kang,G. and Wang,D. TITLE Direct Submission JOURNAL Submitted (20-AUG-2009) Molecular Microbiology and Immunology, Washington University, Campus Box 8230, 660 S. Euclid Ave, Saint Louis, MO 63110, USA COMMENT On Sep 25, 2012 this sequence version replaced gi:261291335. FEATURES Location/Qualifiers source 1..6531 /mol_type="genomic RNA" /country="USA" /isolation_source="stool" /collection_date="2009" /host="Homo sapiens" /db_xref="taxon:683173" /isolate="VA2/human/Stl/WD0680/2009" /organism="Astrovirus VA2" 5'UTR 1..42 gene 43..2706 /gene="ORF1a" CDS 43..2706 /codon_start=1 /protein_id="ACX83590.2" /gene="ORF1a" /translation="MERSYKPGGLNIYDPYDRVLQHGSKSARIKGIQLDKVSGNKLAD IFSDGGPLCFGYGVLEMVKVDTGSVQPVTTPVKTVYVSGVLAGNEYVTYCFKPGVNQW VEVDPDIHQPTALVGTLYQEYKKLVQSERELKETKSGLQLEISLLRHELERSRPTVRT LRPYKMANIVFFGLLLGLLMAHAVTGFRTGQCLDPDVSETLKPQTCINWKWDGGIAPD TEVPLVERAYAWYTGVKQQMKEYYNDSVIVDWSVYVFKMLCSWTSVAVTIGVFYMIKS ENPMYMMLTLMLATLSRIQLMAVAAIPGMEITSAFSLWCCMIVYYFNQAAAMASAIII ASVCYVSCMFMSDIEYVQVLRGHAVVVFTIICSHIFHILQIPSWVTVLIMVLYRVVRL TSVVIGEKIEVRNLDGKVVNTITTQTSWLNKVSKFVQSKFKQNVRVGVSSTARVVPNG VLVVEAKDNIGTGFRVQNYVVTAAHVVGSETQVRLKWGDVSAFAKVVYIHPSKDVAYL SLPPEMQNLPTYKFAKAVADGTIVITSLEDCGVLAVAITEGVVVSNNMTYAVCTKNGM SGSPVTNVDGRVIGVHQTNTGFTGGAVIIRQEDLPPQKKPQRELELEEKVKQLEEALA GKMNQKFSEDQVIELIRMAVGREIGILRHELSMNQAKGKNKGKRRGNVKRKRRRMWTE EEYKELLEKGFTRQQLRDMAETLREAEYTDTESEEYESGYPAWSDPEDSDEIDREWFG PKKKILDEVDSGWSKGDFWEQCQKVWKETEPMSEEQVNTLPSHLHEKYGLTCYVITKA DMEALAKDLLKYQSLVEDKIKNNVVRGQWIDGVDPKVIINELDELWLGINHIMWENGL VPFTQRRKINKRKNQKNLKGGLKVSPQQKNN" /product="putative serine protease" gene <2643..4229 /gene="ORF1b" CDS <2643..4229 /codon_start=1 /protein_id="ACX69838.1" /gene="ORF1b" /translation="KKESKKLEGGAQGEPPTKEQLTLSYWENMLEPGDYYLTPPTYPL LGVLPINRPICDYDEPIDDLLNLLPSYDDDMSYGPTVWGPEAYVKSFEKFTYKEPMSN IKDKYKREWNFAMRVLRREFDYLIDSVMTDITATSKNSDSTPSYPKCLWWKTEAEYLK ERGYQDYITQLESIKKGERPKVLWYLFLKKEILKLSKIEDGDIRQIVCSDPIFARIGC VFEEHQNNLMKMRTKTRMGQCGWSPFCGGFNDHVKRLVDKGNNLFVEFDWTRYDGTIP NEVFMAIKQFRYSCLAEEFKTEENLSIYKWYCESILDRYVMLPSGEVTKQVRGNPSGQ VSTTMDNNLCNVFFQAFEYAYMHPDKDISQLMHDWERVDSLIYGDDRLSTYPELPGDY VDRVVDMYATVFGMWVKPEKVKIPNSIIGLTFCGFTVTESNGVYVPVPTETEKLMAGL VRPTKRLPDILSLYGKLLCYRILSHNLPDDHKFKNYILVALEVVARHIRASGGEEPYY ITDGMLDRLWRGGPKRGDGW" /product="RNA-dependent RNA polymerase" /note="RNAP; translated by ribosomal frameshift" CDS 4219..6414 /codon_start=1 /protein_id="ACX83591.2" /translation="MAGKQPQHAATKAAIKAVAKEVVKEEKKAFKPRQQNKSTRNKYR NKWQTKHQVKNEIKTELKKKGLEGPKTKFTVKVSATIGKIGPNVNSGPELQISTFMHP ALMKEPNDGTNFGPLQAAAAQWGLWRLSDLKIQFTPLVGSSAVTGSVYRTSLNLTQSP GSTSWGGLGARKHLDIPVGVSRTWHLKKGDLAGPRETWWLTDTNEEGGQSCGPMLEVH GLGKTTSTYKDEAWRGDLFIVEVTGVWQFTNYNAKPALGTLNRVVEDTTATIEVGTDG IMKMSIPSTGQLARHMSEQNERASNAANTIGETIWQVVDQGAGLVASVAPEPFGWLIK GGWWFVKKLIGRANTTTEQYYVYASLADAQNNKPVEANTFAASSHATTLAVTQINAPN TGPSTASAVVQSRMFPLPQAGIPDGWFWLSGQFEALHTVGLNGTNGSTLPCGMITSFD KWEFKLKKGTVEWGCAMQGMAAASNTVTCWSVDRENQLLGWYDVSGIASTGIVVDWVT GPNGGTVHLDWADVLAWRTDLWGTLRMTYWLCRTRRAVQGSDFDTTQKSPSPYIARAW PETISNVRLDVREVLMVNTTTNATDSNSRVQYIQRVPAGAIVILWCLGNHTFDTSTGQ GSIVQKSSFGGFGQLPSKSSTTGLWSRALNSVGPSTDWITVSFDSHPPVCDDVVSRLM AEIADRYDLEPRRKKPKDATKELTRLKIYEALREADWEHLPAEEVSSVL" /product="capsid protein" 3'UTR 6415..6531 ORIGIN 1 ccaagtaggg tttggtgcct ttgggcatct ggtgggttag ctatggagcg ttcctataaa 61 ccagggggtc ttaatatcta tgacccttac gaccgtgttt tgcaacacgg aagtaagagt 121 gccagaataa agggtattca actggacaaa gtttcaggga ataagttggc agacatattc 181 tcagatggtg gcccattgtg ctttggttat ggagttctgg aaatggttaa ggttgacact 241 ggttcagtgc aaccagtgac tacaccagtg aaaaccgtgt acgtctcagg cgtccttgcg 301 ggcaatgagt acgttaccta ctgcttcaaa cctggtgtta accaatgggt tgaagtggat 361 ccagatatac atcaaccaac ggctttggtg ggtacattgt atcaagaata caaaaaactg 421 gtccagtctg aaagggaatt gaaagaaact aagtcaggac tacaattaga aatatccctt 481 ttgaggcacg aattggaacg ctccagaccc actgtcagaa ccctacgccc atacaaaatg 541 gctaacatag tgttttttgg attactactg ggattgttaa tggcacatgc agttacaggc 601 tttagaacag gacaatgcct tgacccagat gtaagtgaaa ctctgaagcc tcagacctgt 661 attaattgga aatgggatgg tggtatagct ccagacaccg aggtaccact agttgaaagg 721 gcatatgcat ggtatacagg agttaaacag cagatgaagg aatactataa tgacagcgtt 781 atagtagatt ggtctgtgta tgtgttcaag atgttatgtt catggacatc agttgcagtg 841 actatagggg ttttctacat gattaagtca gaaaatccca tgtatatgat gttaacattg 901 atgctggcta cactatcaag gatacagcta atggcagtgg ctgccatacc tggaatggaa 961 ataacatcag cattttcatt gtggtgctgt atgatagtgt actactttaa tcaagcagct 1021 gcgatggcat ctgcaattat tatagcttca gtgtgttatg tctcttgcat gttcatgagt 1081 gacattgaat atgtacaagt tttgagaggc catgccgtgg ttgtttttac cataatatgc 1141 tcccacattt tccatatttt acaaatacca tcttgggtta ctgttttaat aatggtttta 1201 tacagggtgg tgaggttaac tagtgttgtg attggagaga aaattgaggt taggaacctc 1261 gacggtaagg tggttaatac aatcacaact cagacatcat ggcttaataa agtttccaaa 1321 tttgtgcaaa gtaaattcaa gcaaaatgtt agggtgggtg tttcatcaac tgctcgagtt 1381 gtaccaaatg gggtattagt tgtggaggcc aaggataaca tcgggactgg ctttagagtg 1441 caaaactatg ttgttacagc agcacatgtt gttggtagtg aaacccaggt tcgcctaaaa 1501 tggggtgatg ttagtgcatt tgctaaggtt gtttacatac atcctagtaa ggatgtggca 1561 tatttaagtt tgccacctga aatgcagaac ttaccaacat acaagtttgc aaaagccgtt 1621 gctgatggga ccatagtcat tacctcatta gaggattgtg gggttttggc tgttgccatc 1681 acagaaggtg ttgtcgtctc taacaacatg acatatgctg tgtgtaccaa aaatggtatg 1741 agtgggtcac cagttaccaa tgttgatggg cgcgtcattg gtgttcatca gacaaacact 1801 ggtttcactg gtggcgcagt cattatacgc caggaagacc tcccacccca aaagaagcca 1861 caacgtgagt tggagctgga ggagaaggtt aagcaacttg aggaagccct agcaggaaaa 1921 atgaatcaaa aattcagtga agaccaggtc atcgaattga taaggatggc tgttggtaga 1981 gaaattggaa tattacgcca cgaattgtct atgaatcaag ccaaaggtaa aaataagggt 2041 aagaggcgtg gcaatgtcaa acgtaagagg aggaggatgt ggactgaaga agagtacaaa 2101 gagctcctag aaaaaggctt taccagacag cagctacggg atatggctga aacgctgcgt 2161 gaggcagaat atactgacac tgagagtgag gaatatgagt caggttatcc tgcttggtct 2221 gatccagaag attctgatga gatagaccga gaatggtttg ggcccaaaaa gaagatactt 2281 gatgaggttg actctggatg gtccaaagga gacttctggg agcagtgtca aaaagtgtgg 2341 aaggaaactg aaccgatgag tgaggagcaa gtgaacactc ttccatcaca cctgcatgaa 2401 aaatacggtt tgacatgtta cgtcattaca aaagcagaca tggaggcatt ggctaaagac 2461 ttgctaaagt atcaatcatt ggttgaagat aaaataaaga acaatgtggt caggggccaa 2521 tggattgatg gtgtggatcc caaggtgatc ataaatgaat tagatgaatt gtggcttggt 2581 atcaatcaca taatgtggga gaatgggctt gtcccattta cccaacgccg taagatcaat 2641 aaaagaaaga atcaaaaaaa cttgaagggg gggctcaagg tgagccccca acaaaagaac 2701 aattaactct ttcttattgg gaaaatatgt tggagccagg ggattattac cttacccctc 2761 caacataccc tttattgggt gttttaccaa tcaaccgacc aatatgtgat tatgatgagc 2821 caattgatga tttgctaaat ttgttgccaa gttatgatga tgatatgtca tatggtccaa 2881 cagtttgggg ccctgaagcc tatgttaagt catttgaaaa gtttacatat aaagaaccaa 2941 tgagtaacat caaggacaaa tataagagag agtggaactt tgccatgaga gtcttgagga 3001 gggagtttga ttacctaatt gatagtgtga tgacagacat cacagcaaca tctaagaatt 3061 cagactccac accctcctac ccaaaatgcc tgtggtggaa aacagaggct gaatacttga 3121 aagagagggg ctaccaagat tatataacac agttggaatc cataaagaaa ggtgaaaggc 3181 ccaaagttct ctggtatttg tttctcaaga aggagatcct aaagctcagc aaaattgagg 3241 atggggatat acgacaaatc gtgtgttcag atccgatatt tgcgcgcatt gggtgtgttt 3301 ttgaggaaca tcagaataac ctaatgaaaa tgaggacaaa gacccgcatg gggcaatgtg 3361 gctggtcccc attctgtggt ggcttcaatg atcatgtcaa gaggctggtg gataaaggta 3421 acaatttgtt tgtcgagttt gattggacac gatatgatgg tacaatacca aatgaagtgt 3481 ttatggcaat taaacaattt aggtactcat gtttagctga ggagtttaaa acagaggaga 3541 atttgagtat ctataaatgg tattgtgaga gtatactaga taggtacgtt atgttacctt 3601 ctggtgaagt caccaaacag gttaggggaa acccttcagg tcaagtgtca acaactatgg 3661 acaataattt gtgtaatgtc ttcttccaag cgtttgagta cgcgtacatg caccctgata 3721 aggatataag tcaacttatg catgattggg agagggttga ttcacttata tatggtgatg 3781 atagattgtc tacttatcct gagttgcctg gagattatgt agacagagtt gttgatatgt 3841 atgccacagt gtttggaatg tgggtcaaac ctgaaaaagt gaaaatccca aatagtataa 3901 taggccttac attttgtggt ttcacagtga cagaatcaaa tggggtatat gtcccagtcc 3961 ccacagaaac agagaaactc atggctggac tagtgcgacc aactaaaaga ttacctgaca 4021 ttttatcgct gtatgggaaa ctcctttgct accgcatact aagtcacaat ctgccagatg 4081 accacaaatt taaaaattac atcttggtgg ccttagaagt tgtggctagg cacatccgtg 4141 ctagtggagg tgaagagcct tactatatta cggatggtat gctggataga ctttggaggg 4201 gcggaccaaa gcgtggagat ggctggtaag cagccccagc atgctgcaac taaggctgcc 4261 attaaggcag ttgctaaaga ggttgtcaag gaggagaaga aggcattcaa acccagacag 4321 caaaacaaaa gcactaggaa taagtataga aataaatggc aaactaaaca tcaagttaag 4381 aatgaaatta agactgaact taagaagaaa ggacttgagg gacccaagac aaaatttaca 4441 gttaaagtct cagcaacaat tggaaaaatt ggaccaaatg taaattcagg gcctgaatta 4501 caaatttcaa catttatgca tcctgctttg atgaaagagc caaatgatgg caccaacttt 4561 ggcccattgc aagctgcagc tgctcagtgg ggtctgtgga gactatcaga cctcaagata 4621 cagtttacac cactcgtggg ttcatcggct gtaactggct cagtgtaccg cacatctttg 4681 aacctaacgc aatcccctgg gtctacatct tggggtggtc taggtgcccg aaaacacctg 4741 gacattccag ttggggtgtc acggacatgg cacctaaaga aaggtgatct tgctgggccc 4801 agggagacat ggtggttaac agataccaat gaagagggag ggcaaagctg cggtcctatg 4861 cttgaagtcc atggcctcgg caaaacaacc tcaacttata aggatgaagc ctggaggggt 4921 gatctcttta tagttgaagt tacaggagtg tggcagttca caaactacaa tgccaaacct 4981 gctttgggaa cactgaacag ggtggttgag gacaccacgg cgactattga ggtcggcaca 5041 gatggcataa tgaagatgtc aataccatca actgggcagc tagcacgcca catgtcagag 5101 caaaatgaaa gggcatcaaa tgcagcgaac acaattggtg agaccatatg gcaggtcgtt 5161 gaccaaggag ctggtcttgt tgccagtgtt gcacctgagc ctttcggctg gctcatcaag 5221 ggtggttggt ggtttgttaa gaaactgatt ggcagggcca ataccactac tgaacagtat 5281 tatgtttatg cttcactagc agatgcacaa aacaacaaac cagttgaagc aaatactttc 5341 gctgcttcaa gccatgctac aacactggct gttactcaaa taaatgctcc taacactggg 5401 ccaagcacag cctcagcggt tgtccaatcc agaatgtttc ccctaccaca agcggggatt 5461 cctgatggtt ggttttggct cagtggacaa tttgaagctt tgcacacggt tggtttgaat 5521 ggtaccaacg ggtctactct tccatgtggt atgatcacat catttgacaa atgggagttc 5581 aaactaaaga aaggcactgt tgagtgggga tgtgctatgc aaggaatggc agcagcaagc 5641 aatactgtta cttgctggtc agttgatcgt gaaaatcagc ttttgggttg gtacgatgtc 5701 agcggcattg cttcaactgg cattgtcgtt gattgggtaa ctggcccaaa tggtggcacc 5761 gtgcatttgg actgggctga tgtccttgct tggaggacag atttgtgggg gacactccgc 5821 atgacatact ggttgtgtcg aactagaaga gccgtgcaag gctctgactt tgatacgaca 5881 cagaagagcc catcgcctta catcgcgcgg gcttggcctg agacaataag caatgtcagg 5941 cttgatgttc gggaggtttt gatggttaat acaaccacca atgccactga ttcaaatagc 6001 cgagtccaat atatccaaag agtgccagct ggcgccatag tcatactctg gtgcctgggg 6061 aatcacacat ttgacacatc aactggccaa ggttccattg tccaaaagtc ctcatttggt 6121 ggatttggac agctgcctag taagtcatct actactggtt tgtggtctcg tgcacttaat 6181 tcagttggtc catctaccga ttggattact gtgtcttttg actcacaccc accagtttgt 6241 gatgacgttg tttcaaggct gatggctgaa attgctgaca ggtatgacct tgagccccgc 6301 agaaagaagc ctaaggatgc aactaaagag ctaaccaggc tcaaaatcta tgaagcactt 6361 agagaagctg attgggaaca cctacctgca gaggaggtga gctcagtgct ttaaattccg 6421 ccgaggccac gccgagtagg atcgagggta cagcggacta ttgattgctt gtggaatgaa 6481 ttagtttatg attataatct gttcatttga tcattagtga atttgattct c //