LOCUS KU059752 15179 bp cRNA linear VRL 25-MAR-2017 DEFINITION Newcastle disease virus isolate TX3988, complete genome. ACCESSION KU059752 VERSION KU059752.1 DBSOURCE BioProject accession PRJNA257008 KEYWORDS . SOURCE Avian avulavirus 1 ORGANISM Avian avulavirus 1 Viruses; ssRNA viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Avulavirus. REFERENCE 1 (bases 1 to 15179) AUTHORS Tesh,R.B., Bukreyev,A., Forrester,N.L., Lin,D., Hari,K. and Weaver,S.C. CONSRTM Consortium for Microbial Forensics and Genomics (microFORGE) TITLE Direct Submission JOURNAL Submitted (02-NOV-2015) Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: Trimmomatic v. 0.32; SGA v. 0.10.13; iMetAMOS v. 1.5; samtools v1.1; FastQC v. 0.10.0; Spades v. 3.1.1; idba v1.1.1; Pilon v. 1.8; Quast v. 2.2; Prokka v. 1.7 Assembly Name :: NDV002-SEQ-2-ASM-1 Genome Coverage :: 1092.34x Sequencing Technology :: Illumina HIseq1500 ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..15179 /mol_type="viral cRNA" /country="USA: Texas, Harris County, Houston" /collection_date="10-Dec-2004" /host="Streptopelia decaocto" /db_xref="taxon:11176" /isolate="TX3988" /organism="Avian avulavirus 1" gene 115..1584 /locus_tag="VD97_00001" /gene="N" CDS 115..1584 /locus_tag="VD97_00001" /protein_id="AMZ00498.1" /gene="N" /codon_start=1 /inference="ab initio prediction:Prodigal:2.60" /inference="similar to AA sequence:UniProtKB:Q99FY3" /product="nucleocapsid protein" /translation="MSSVFDEYEQLLAAQTRPNGTHGGGEKGSTLKVEVPVFTLNSDD PEDRWNFAVFCLRIAVSEDANKPLRQGALISLLCSHSQVMRNHVALAGKQNEATLAVL EIDSFNNSVPQFNNRSGVSEERAQRFMVIAESLPRACSNSTPFVTAGVENDVPEDITD TLERILSIQAQVWVTVAKAMTAYETADESETRRINKYMQQGRVQKKYILHPVCRSAIQ LTIRHSLAVRIFLVSELKRGRNTAGGSSTYYNLVGDVDSYIRNTGLTAFFLTLKYGIN TKTSALALSSLTGDIQKMKQLMRLYRMKGENAPYMTLLGDSDQMSFAPAEYAQLYSFA MGMASVLDKGTGKYQFARDFMSTSFWRLGVEYAQAQGSSINEDMAAELKLTPAARRGL AAAAQRVSEEIGNVDIPTQQAGVLTGLSDEGPRAPQSGPNRSQGQPDARDGETQFLDL MRAVANSMREAPDSAQSTIHPEPLPTHGPSQDNDTDWGY" gene 1886..3073 /locus_tag="VD97_00002" /gene="P/V" CDS 1886..3073 /locus_tag="VD97_00002" /protein_id="AMZ00499.1" /gene="P/V" /codon_start=1 /inference="ab initio prediction:Prodigal:2.60" /inference="similar to AA sequence:UniProtKB:Q06427" /product="phosphoprotein" /translation="MATFTDAEIEDIFETSGTVIDSIITAQGKSAETVGKSAIPQGKT KALSIAWEKHGSIQPSADQDTSNQQDKPDKQPSTSEQATPHNDPLTASKEPLPTQAAG DAGGTQLKTGASNSLLSMLDKLSNKSSNAKKGPRSSPQEGHHQPLAQQHGNQSSRGNN QEGPLHQAKAAPGSRGTDVSTAYHGQWKESQLSAGATPHVLQSGRSQDSTPVPVEHVQ LPVDFVQAMMSMMEALSQKVSKIDYQLDLVSKQTSSIPMMRSEIQQLKTSVAIMEANL GMMKILDPGCANVSSLSDLRAVARSHPVLVSGPGDPSLYVTQKGEMTLNKLSQPVQHP SELIKSAMVSGPDMGVEKETVRALITSRPMHPSSSAKLLRKLDAAKSVEEIRKVKRLA LNG" gene 3289..4383 /locus_tag="VD97_00003" /gene="M" CDS 3289..4383 /locus_tag="VD97_00003" /protein_id="AMZ00500.1" /gene="M" /codon_start=1 /inference="ab initio prediction:Prodigal:2.60" /inference="similar to AA sequence:UniProtKB:P11206" /product="matrix protein" /translation="MDSSRTIGLYFDSALPSSSLLAFPIVLQDTGDGKKRITPQYRIQ RLDSWTDSKEDSVFITTYGFIFQIGNEEVTVGTINDNPRHELLSSAMLCLGSVPNDGD LVELARACLTMAITCKKSATNTERIVFSVVQAPRVLQSCMVVANRYSSVNAVKHVKAP EKIPGSGTLEYKVNFVSLTVVPRKDVYKIPTAALKISGSSLYNLALNVTIDVEVDPKS PLVKSLSKSDSGYYANLFLHIGLMSTVDKKGKKVTFDKIEEKIRRLNLSVGLSDVLGP SVLVKARGARTKLLAPFFSGSGTACYPIANASPQVAKILWSQTAHLRSVKVIIQAGTQ RAVAATADHEVTSTKIEKRHTIAKYNPFKK" gene 4543..6204 /locus_tag="VD97_00004" /gene="F" CDS 4543..6204 /locus_tag="VD97_00004" /protein_id="AMZ00501.1" /gene="F" /codon_start=1 /inference="ab initio prediction:Prodigal:2.60" /inference="similar to AA sequence:UniProtKB:P14623" /product="fusion glycoprotein F0 precursor" /translation="MGSKPSTRILAPLMLITRITLILSCICLTSSLDGRPLAAAGIVV TGDKVINIYTSSQTGSIIVKLLPNMPKDKEACAKAPLEAYNRTLTTLLTPLGDSIRRI QGSVSTSGVRRKKRFIGAIIGSVALGVATSAQITAAAALIQANQNAANILRLKESIAA TNEAVHEVTDGLSQLAVAVGKMQQFVNDQFNNTARELDCIKIAQQVGVELNLYLTELT TVFGPQITSPALTQLTTQALYNLAGGNMDYLLTKLGIGNSHLSSLIGSGLITGNPILY DSQTQILGIQVNLPSVGNLNNMRATYLETLSVSTTKGFASALVPKVVTQVGSVIEELD TSYCIESDLDLYCTRIVTFPMSPGIYSCLSGNTSACMYSKTEGTLNTPYMALKGSVIA NCKITTCRCADPPGIISQNYGEAVSLIDRHSCNVLSLDGITLRLSGEFDATYQKNISI LDSQVIVTGNLDISTELGSVNNSISNALDRLAESNSKLDKVNVKLTSTSALITYIVLT VISLVFGTLSLVLACYLMYKQKAQQKTLLWLGNNTLDQMRATTRT" gene 6411..8126 /locus_tag="VD97_00005" /gene="HN" CDS 6411..8126 /locus_tag="VD97_00005" /protein_id="AMZ00502.1" /gene="HN" /codon_start=1 /inference="ab initio prediction:Prodigal:2.60" /inference="similar to AA sequence:UniProtKB:P12558" /translation="MDHAVSKVALENEEREAKNTWRLVFRIAVLLLIVVTLAISAAAL VYSAGASTPRDFVGISTVVSKVEDKITSLLSSNQDVVDRIYKQVALESPLALLNTESI IMNAITSLSHQINGAANNSRCGAPVHDPDYIGGIGKELIVDDISGVTSFYPSAYQEHL NFIPAPTTGSGCTRIPSFDMSATHYCYTHNVILSGCRDHSHSHQYLALGVLRTSATGR VFFSTLRSINLDDTQNRKSCSVSATPLGCDILCSKVTEAEEEDYKSVTPTSMVHGRLG FDGQYHEKDLDITVLFKDWVANYPGVGSGSFIDDRVWFPVYGGLKPNSPSDTAQEGKY VIYKRYNNTCPDGQEYQIRMAKSSYKPGRFGGKRVQQAILSIKVSTSLGEDPVLTVPP NTITLMGAEGRVLTVGTSHFLYQRGSSYFSPALLYPMTVYNKTATLHSPYTFNAFTRP GIVPCQASARCPNPCITGVYTDPYPLVFYRNHTLRGVFGTMLDDEQERLNPVSAVFDN ISRSRVTRVSSSSTKAAYTTSTCFKVVKTNKVYCLSIAEISNTLFGEFRIVPLLVEIL KDDRV" /product="hemagglutinin-neuraminidase" /EC_number="3.2.1.18" gene 8380..14994 /locus_tag="VD97_00006" /gene="L" CDS 8380..14994 /locus_tag="VD97_00006" /protein_id="AMZ00503.1" /gene="L" /codon_start=1 /inference="ab initio prediction:Prodigal:2.60" /inference="similar to AA sequence:UniProtKB:P12576" /product="transcriptase" /translation="MASSGPERAEHQIILPESHLSSPLVKHKLLYYWKLTGLPLPDEC DFDHLIISRQWKKILESATPDIERMIKLGRAVHQTLNHNSKIIGVLHPRCLEELSSIE VPDSTNKFRKIEKKIQIHNTRYGDLFARLCTHVEKKLLGSSRSSKVPRSEEFSSIRTD PAFWFHSKWSRAKFAWLHIKQIQRHLIVAARTRSAVNKLVTLTHKIGQVFVTPELVIV THTDENKFTCLTQELVLMYADMMEGRDMVNIISSTATHLRNLSEKIDDILRLVDALSK DLGNQVYDVVALMEGFAYGAVQLLEPSGTFAGDFFAFNLQELKDTLIEHLPNDIVESV THAIASIFSGLEQNQAAEMLCLLRLWGHPLLESRSAAKAVRSQMCAPKMIDFDMILQV LSFFKGTIINGYRKKNSGVWPRVKIDTIYGKVIGQLHADSAEISHDVMLREYKSLSAL EFEPCIDYDPVTNLSMFLKDKAIAHPKDNWLASFRRNLLSEDQKKQVKEATSTNRLLI EFLESNDFDPYREMEYLTTLEYLRDDSVAVSYSLKEKEVKVNGRIFAKLTKRLRNCQV MAEGILADQIAPFFQGNGVIQDSISLTKSMLAMSQLSFNSNKKRITDCKERVSSNRNH DPKSKNRRRVATFITTDLQKYCLNWRYQTVKLFAHAINQLMGLPHFFEWIHLRLMDTT MFVGDPFNPPSDPTDCDLSRVPNDDIYIVSARGGIEGLCQKLWTMISIAAIQLAAARS HCRVACMVQGDNQVIAVTREVRSDDSPDMVLTQLHQASDNFFKELIHVNHLIGHNLKD RETIRSDTFFIYSKRIFKDGAILSQVLKNSSKLVLISGDLSENTVMSCANIASTVARL CENGLPKDFCYYLNYLMSCVQTYFDSEFSITHSSQPDSTQSWIEDISFVHSYVLTPAQ LGGLSNLQYSRLYTRNIGDPGTTAFAEVKRLEAVGLLSPSIMTNILTRPPGNGDWASL CNDPYSFNFETVASPNIVLKKHTQKVLFETCSNPLLSGVHTEDNEAEEKALAEFLLNQ EAVHPRVAHAIMEASSVGRRKQIQGLVDTTNTVIKIALTRRPLGIKRLTRIINYSSMH AMLFRDDVFLPNRSNHPLVSSAMCSLTLADYARNRSWSPLTGGRKILGVSNPDTIELV EGEILSVSGGCTKCDSGDEQFTWFHLPSNIELTDDTSKNPPMRVPYLGSKTQERRAAS LAKIAHMSPHVKAALRASSVLIWAYGDNEVNWTAALKIARSRCNISSEYLRLLSPLPT AGNLQHRLDDGITQMTFTPASLYRVSPYIHISNDSQRLFTEEGIKEGNVVYQQIMLLG LSLIESLFPMTTTKTYDEITLHLHSKFSCCIREAPVAVPFELLGLAPELRTVTSNKFM YDPSPISERDFARLDLAIFKSYELNLESYSTMELMNILSVSSGKLIGQSVVSYDEDTS IKNDAIIVYDNTRNWISEAQNSDVVRLFEYAALEVLLDCSYQLYYLRVRGLNNIVLYM SDLYKNMPGILLSNIAATISHPIIHSRLNAVGLVNHDGSHQLADTDFIEMSAKLLVSC TRRVVSGLYAGNKYDLLFPSVLDDNLSEKMLQLISRLCCLYTVLFATTREIPKIRGLS AEEKCSVLTEYLLSDAVKPLLKSEQVSSIVSPNIITFPANLYYMSRKSLNLIREREDR DAILTLLFPQEPLFELRPVQDIGARVRDPFTQQPASFIQELDLSAPARYDAFTLSEVH FEHTLPNPGEDYLVRYLFRGIGTASSSWYKVSHLLSVPEVRCARHGNSLYLAEGSGAI MSILELHIPHETIYYNTLFSNEMNPPQRHFGPTPTQFLNSVVYRNLQAEVPCKDGFVQ EFCPLWRENAEESDLTSDKAVGYITSVVPYRSVSLLHCDIEIPPGSNQSLLDQLATNL SLIAMHSVREGGVVIIKVLYVMGYYFHLLMNLFTPCSTKGYILSNGYACRGDMECYLV FVMGYLGGPTFVNEVVRMAKTLIQQHGTLLSKSDEIALTKLFTSQQHRVVDILSSPLP RLMKFLRENIDAALIEAGGQPVRPFCAESLVGTLADMTQMAQIIASHIDTVIRSVIYM EAEGDLADTVFLFTPYNLSTDGKKRTSLKQCTRQILEVTILGLRARDLNKVGDVIGLI LRGTVSLEDLIPLRTYLKHSTCPKYLKAVLGITKLKEMFTETSLLYLTRAQQKFYMKT IGNAAKGYYSNNDS" ORIGIN 1 agagaatctg tgagttacgg caaaaggcga agaagcaatc gaaatcgcac gggtagaagg 61 tgtgaacctc gagtgcgagg ccgaaactca aatctgaggg aactttctgc cggcatgtcg 121 tctgtctttg acgagtacga gcagctcctc gctgctcaga ctcgccctaa tggaactcac 181 ggaggaggag agaaagggag cactttaaaa gttgaggtcc cagtattcac tcttaacagt 241 gatgatccag aagatagatg gaattttgcg gtattctgtc ttcggattgc tgttagtgag 301 gatgccaaca aaccgctcag gcaaggtgct ctcatatccc tcttatgctc ccattctcaa 361 gtgatgagga accatgttgc cctcgcaggg aaacagaatg aggccacact ggctgttctt 421 gagatcgaca gttttaataa cagtgtgccc cagttcaaca acaggagcgg agtgtctgaa 481 gagagagcac agagattcat ggtgatagca gagtctctcc ctcgggcatg cagtaacagt 541 actccgttcg tcacagctgg agttgaaaat gatgtaccag aagatatcac tgacactctg 601 gaaagaatct tatctatcca ggctcaggta tgggtcacag tagcaaaagc catgactgca 661 tatgagacag cagatgagtc agaaacaaga aggatcaata agtatatgca gcaaggtaga 721 gtccagaaga agtacatcct ccatcctgta tgcaggagtg caattcaact tacaatcaga 781 cattcactag cagtccgcat ttttctagtt agtgagctta agagaggccg caatacagca 841 ggtgggagct ccacgtatta caacttagtt ggggatgtag actcatacat aaggaacacc 901 gggcttaccg cattcttcct gacactcaag tatggaatca ataccaagac atcagccctt 961 gcactcagca gcctcacagg tgatatccaa aaaatgaaac agctcatgcg tttataccgg 1021 atgaaaggag aaaatgcacc gtacatgaca ttgctaggtg atagtgacca gatgagcttc 1081 gcaccagctg aatatgcaca actctattcc tttgccatgg gcatggcatc ggtcttagac 1141 aagggaactg gtaaatacca attcgccaga gacttcatga gcacatcatt ctggagactc 1201 ggggtggagt atgctcaggc tcaggggagt agcatcaatg aggatatggc tgctgagctg 1261 aaactaactc cagcagcaag gaggggtctg gcagctgctg cccaacgagt atctgaggaa 1321 attggcaatg tggatattcc tactcaacag gccggggtcc tcaccgggct cagcgacgaa 1381 ggtccccgag ctccgcagag tggaccgaac aggtcgcaag ggcaaccaga cgccagggat 1441 ggggagaccc aattcttgga cctgatgaga gcagtggcaa acagcatgcg agaagcacca 1501 gactctgcac agagcaccat ccacccggag cccctcccaa ctcatgggcc atctcaggac 1561 aacgataccg actgggggta ctgatcgaca acattcagcc tgccttcaca gggtcatccc 1621 aaatcctccg ccgaaacctc ccccaaactc cccgacccgc cggcccacac ggccaaatcg 1681 gcaggagaat ccccccaacc cctcccccac ccccagccgc acgaccccac ccgcccagag 1741 caacacgggc acaattcaat tcaccaacaa tccgtgtaga acccgaaata ttagaaaaaa 1801 atacgggtag aagagagaca ctcagagatc aggacgagtc accaaggtct ctactcttcc 1861 ctctgcccag tgaactaggg tgaagatggc cacttttacc gatgctgaga tagaggatat 1921 atttgagacc agtggaactg tcattgacag cataattacg gcccagggca aatcagcaga 1981 gaccgtcggg aagagtgcaa tcccgcaagg caagacaaag gccctgagca tagcatggga 2041 gaagcatggg agcatccagc cgtccgctga ccaggacacc tctaaccaac aggacaaacc 2101 agacaagcag ccatccacat ctgagcaggc gactccacac aatgaccctc tgactgcgtc 2161 caaagaaccg ctccccactc aggccgcagg tgatgccggc ggtacacagc tcaagaccgg 2221 agcaagcaac tctcttttgt cgatgcttga caagctgagc aacaagtcgt ctaatgctaa 2281 aaaggggcca aggtcgagcc cccaggaggg ccaccatcaa cctctggccc aacaacacgg 2341 gaatcagtcg agccgtggaa acaaccagga aggaccgctg caccaggcca aagctgcccc 2401 tggaagccgg ggcacagacg tgagcacagc atatcatgga caatggaagg agtcacaact 2461 atcagctggt gcaacccctc atgtgctcca gtcagggcgg agccaagaca gtactcctgt 2521 acctgtggag catgtccaac tacctgtcga ctttgtgcag gcgatgatgt ctatgatgga 2581 ggcgttgtca cagaaggtaa gtaaaattga ctatcagcta gacctagtct caaagcagac 2641 atcctccatc cctatgatgc gatctgaaat ccaacagctt aaaacatcgg ttgcaatcat 2701 ggaagccaat ttaggcatga tgaaaattct ggatcctggt tgtgctaacg tttcgtcctt 2761 aagtgatcta cgggcagtcg ctcgatccca cccagtttta gtttcgggcc ccggagatcc 2821 atccctttac gtgacacaaa agggtgaaat gacactcaat aaactctcgc aaccggtaca 2881 gcacccttct gagttaatta aatctgccat ggtaagcggg cccgacatgg gagtggagaa 2941 ggaaactgtc cgtgcattaa ttacatcgcg cccgatgcat ccaagctcct cggctaagct 3001 tctgaggaag ttggacgcag ccaagtcagt cgaagagatc agaaaggtca agcgtcttgc 3061 gctgaacggt tgacagccat cacagtccac gacaggctcc tgcctcacca gcgccacaaa 3121 gaatccctcc cgaaccttcc cccacagacc cgcgccccaa caccccaagc aacgactctc 3181 tccctcctct ctcactcccc cgagtgatcg cgcagctgca atcaatctac cagcattaga 3241 gattaagaaa aaatacgggt agaataaaag taccccaact gtgccaaaat ggactcatcc 3301 agaacaatcg ggctgtactt tgattctgcc ctaccttcca gcagcctgtt agcattcccg 3361 atcgtcctac aagacacggg agatgggaag aagcgaatca ccccacaata caggatccag 3421 cgtcttgact cgtggacaga tagcaaggaa gactcagtat ttatcaccac ctacggattc 3481 atcttccaaa ttgggaatga ggaggtcact gtaggcacga tcaatgacaa tcccagacac 3541 gagttacttt cctctgcaat gctctgccta ggaagtgtcc cgaatgacgg agatcttgtt 3601 gaactggcga gggcctgcct cactatggcg ataacttgca agaagagtgc aactaatact 3661 gagagaatag tcttctcagt agtgcaggca cctcgggtgc tgcaaagctg tatggttgtg 3721 gcaaataggt actcatcagt gaatgcagtg aagcatgtga aagcacccga gaagatcccc 3781 ggaagcggaa ccctagagta taaagtgaat tttgtctcct tgactgtagt gccgaggaag 3841 gatgtctaca agatcccaac tgcagcattg aaaatctctg gctccagtct gtacaatctc 3901 gcgctcaatg tcactattga cgtggaggta gacccgaaga gcccgctagt caaatccctt 3961 tctaagtctg atagtggata ctatgctaat ctttttttgc atatcgggct tatgtccact 4021 gtagataaga agggaaagaa agtgacattt gacaagatag aggaaaagat aaggagactc 4081 aatctttctg tcggactcag tgatgtgctc ggaccttctg tgcttgtgaa ggcgagaggt 4141 gcacggacta agctactggc accttttttc tctggcagcg ggacagcctg ctatcctata 4201 gcaaatgcct ctcctcaggt tgctaagata ctctggagtc aaactgcgca cctgcggagt 4261 gtaaaagtca tcattcaagc tggcacccaa cgtgctgtcg cagcgactgc tgatcatgag 4321 gttacctcta ctaagataga aaagaggcac accattgcta agtacaatcc ttttaaaaag 4381 taagttgcat ctctgagact gtgagccacc cactttccta aatcactgcg acaccagata 4441 atgatatatc ttgattgctt atagttagtt tacctgtcta tctaattaga aaaaacacgg 4501 gtagaagagt ctggatcccg gctagcacat tcaaagtgca atatgggctc caaaccctcc 4561 accaggatcc tggcacctct gatgctgatc acccgaatta ctctgatact gagctgcatc 4621 tgcctgacga gttctcttga tggcaggcct cttgcagctg cggggattgt ggtaacagga 4681 gataaagtga tcaatatata cacctcatct cagacagggt caatcatagt caagttgctc 4741 ccgaatatgc ccaaggacaa agaggcatgt gcaaaagccc cgttagaggc atacaacaga 4801 acactgacca ctttactcac cccccttggt gattccatcc gcaggataca agggtctgtg 4861 tccacatcag gagtaaggag gaagaagcgc tttataggtg ccattatagg cagtgtagct 4921 cttggggttg caacatcggc acagataaca gcggctgcgg ccctaataca agccaatcag 4981 aatgctgcca acatcctccg gcttaaggag agcatcgctg caaccaatga agctgtgcac 5041 gaggtcaccg acggattatc gcaactagca gtagcagttg ggaagatgca acagtttgtc 5101 aatgaccaat ttaataatac agcgcgagaa ttggactgta taaaaattgc acaacaagtc 5161 ggtgtagaac tcaacctata cctaactgaa ctgactacag tgttcgggcc acaaattact 5221 tcccctgctc taactcagct gaccacccaa gcgctttaca atttagctgg cggcaacatg 5281 gattacttgt tgactaaatt aggtataggg aacagtcatc tcagctcatt aataggtagt 5341 ggcttgatca caggcaaccc tatactgtat gactcacaga ctcaaatcct gggcatccag 5401 gtaaatttac cttcagtagg gaaccttaat aatatgcgtg ccacctactt ggagacttta 5461 tctgtaagca caactaaagg gtttgcctca gcacttgtcc cgaaggtagt gacacaagtc 5521 ggctctgtga tagaagaact cgacacctca tactgtatag agtctgatct ggatttatac 5581 tgtacaagaa tagtgacatt ccccatgtct ccaggtattt attcttgtct gagcggcaat 5641 acatcagcct gcatgtactc aaagactgaa ggcacactca atacgccata tatggccctc 5701 aaaggctcag tcattgccaa ttgcaaaata acaacctgca ggtgtgcaga ccccccgggt 5761 atcatatcgc aaaactatgg agaagctgta tctctgatag atagacattc atgcaatgtc 5821 ttatcattag atgggataac tctgaggctc agcggggagt ttgatgcaac ttatcaaaag 5881 aatatctcaa tactagattc tcaagtcatc gtgacaggca accttgatat atcaactgag 5941 cttggaagtg tcaacaattc aataagcaat gccctggaca ggttagcaga aagtaacagc 6001 aaattagaca aagtcaatgt caaattaacc agcacatctg ctctcattac ctatatcgtt 6061 ctaactgtca tatctcttgt tttcggtaca cttagcttgg ttctagcatg ctacttgatg 6121 tacaaacaaa aggcacaaca aaagacttta ctatggcttg ggaacaatac cctcgatcag 6181 atgagagcca ctacaagaac atgaatgcag acgagatgca aatgtgtccc caataataac 6241 ttgtgtgtta attctgacaa cctgttcaat ggaagattta agaaaaaact actggatata 6301 agcaaccaaa aagcaataca cgggtagaac ggtcggagaa gccatcttcc aatcaggaac 6361 taggcttcac aacatctgtt ccaccacatc accaataaca gatttcagct atggaccatg 6421 cagtcagcaa ggttgcgtta gagaatgaag aaagagaagc aaagaacact tggcgcttag 6481 tattccggat cgcagttttg cttttgatag tggtgacctt agctatctct gcagctgccc 6541 tggtatacag tgcgggggct agtacgcccc gcgacttcgt aggcatatcg actgtggtct 6601 ctaaggtgga ggacaagatt acgtctttac tcagttcaaa tcaagatgta gtagatagaa 6661 tatataaaca agtggccctt gaatctccgc tggcactcct aaatactgaa tctataatca 6721 tgaatgcaat aacgtctctc tctcatcaaa tcaatggggc tgcaaacaat agcaggtgcg 6781 gggcgcccgt tcatgaccca gactacatcg gggggatagg caaagaactc atagtagacg 6841 acatcagtgg tgtcacatca ttctacccat ctgcatacca agaacacctg aattttatcc 6901 cggcgcccac tacaggatca ggttgcactc ggataccctc atttgatatg agtgctactc 6961 actactgtta tactcacaac gtgatattgt ctggctgcag agatcactca cactcacatc 7021 aatacttagc acttggtgtg cttcggacat ctgcaacagg gagggtattc ttttctactc 7081 tgcgttccat caatttagat gacacccaaa atcggaagtc ttgcagtgtg agtgcaactc 7141 ctctaggctg tgatatactg tgctctaaag tcacagaggc tgaagaagag gattataagt 7201 cagttacccc aacatcaatg gtgcacggaa ggttaggttt tgacggtcaa taccatgaga 7261 aggacttaga catcacagtc ttatttaagg attgggtggc aaattacccg ggagttggga 7321 gcggatcttt tattgatgac cgtgtatggt tcccagttta tggagggtta aaacctaatt 7381 cgcccagcga cactgcacaa gaagggaaat atgtaatata caagcgctat aacaacacat 7441 gccccgacgg acaagaatat caaatccgga tggctaagtc ttcgtacaag cctgggcgat 7501 tcggtggaaa gcgtgtacag caagccatct tatccatcaa ggtgtcaaca tctttaggtg 7561 aggacccggt gctgactgtt ccgcctaata caattacact catgggggcc gaaggcagag 7621 ttctcacagt agggacatct cacttcttgt accaacgagg gtcttcgtat ttctcccctg 7681 ccttattata tcctatgaca gtctataaca aaacagctac tcttcatagt ccttatacat 7741 ttaatgcttt cacccggcca ggtattgtcc cttgtcaggc atcagcaaga tgccccaatc 7801 catgtatcac tggagtctat acggatccgt accctttggt cttctatagg aatcatacct 7861 tgcgaggggt cttcggaaca atgcttgatg atgaacaaga aagactcaat cccgtatctg 7921 cagtattcga caacatatct cgcagtcgtg taacccgagt gagttcaagc agcaccaagg 7981 cagcatacac gacatcaaca tgctttaaag ttgtcaagac caataaagtt tattgcctta 8041 gcattgcaga aatatctaac accctatttg gggaattcag aatcgttcct ttattagttg 8101 agattcttaa ggatgatagg gtttaagaag ctagatctgg ctggttgagt cgaatggagg 8161 atggttgggg ggatgatgct gcatcaccta tctctcgcaa taccaaggat caaactggat 8221 atcaatgcaa gcctgaatcc cgtaccatca ggcgggcaca acccgacaat gctgatgtga 8281 tcagtctgaa ttttgtccgc agtcgcttta ttaagaaaaa atatggatgg tagtgagata 8341 caagggaaaa caacatacaa aggacagcac gggtaggaaa tggcaagctc cggtcccgaa 8401 agggcagagc atcagatcat cctaccagag tcacatctat cctctccatt agtcaagcac 8461 aaactgctct actactggaa attaactggg ttaccgctcc ctgacgaatg cgactttgat 8521 catctcatta tcagcaggca atggaagaaa atacttgaat ctgccactcc tgacattgag 8581 aggatgataa aactcgggcg ggcagtgcat cagactctca accacaattc caagataatc 8641 ggagtactcc accccaggtg tttagaagaa ctgtcgagta ttgaggtccc tgattcaacc 8701 aacaagtttc ggaagattga aaagaagatt caaattcaca acacgaggta tggagacctg 8761 ttcgccaggc tgtgcacaca cgttgaaaag aaattgctag gatcgtcccg atctagtaaa 8821 gtcccacgat cagaggagtt cagcagcatc cgtacagatc cagcattctg gtttcactca 8881 aaatggtcca gagccaaatt tgcatggctc catataaaac agatccagag gcatctgatt 8941 gtagcagcaa gaacaaggtc tgcagtcaac aagttagtga cattaaccca taagataggc 9001 caagtctttg ttactcctga gcttgtcatt gtgactcata cagatgagaa caagttcaca 9061 tgcctcaccc aagaacttgt attaatgtat gcagatatga tggagggcag agacatggtc 9121 aacataatat catctacagc gacacatctt agaaatctat cagagaaaat tgatgatatt 9181 ctgcggttag tagatgctct gtcgaaggat ttgggcaatc aagtctatga tgtcgtagca 9241 ttgatggagg gatttgcata tggtgctgtt cagctacttg agccgtcagg tacattcgca 9301 ggagatttct ttgcattcaa cctacaagag ctcaaagaca ctctaatcga acacctccca 9361 aatgatatag tggaatcagt aactcacgca atcgcctcca tattctctgg cttagaacag 9421 aatcaagcag ctgagatgtt gtgcttgttg cgtttgtggg gtcacccact acttgagtcc 9481 cgtagtgcag cgaaagcagt caggagccag atgtgcgcac ctaagatgat agacttcgat 9541 atgatcctcc aagtattatc cttctttaag ggaacgatca tcaatggata caggaagaag 9601 aattcaggtg tgtggccacg agtcaagata gatacgatat atgggaaggt cattgggcag 9661 ctacatgctg actcagcaga gatttcacat gatgtcatgt tgagagagta caagagttta 9721 tccgcccttg aatttgagcc atgtatagat tatgaccctg tcaccaatct aagtatgttc 9781 ttaaaggaca aggcaatcgc acatccgaaa gataactggc tcgcctcatt taggcggaac 9841 cttctctctg aggatcagaa gaaacaggta aaggaggcaa cctcaactaa ccgtctcttg 9901 atagagttct tagaatcaaa tgactttgat ccatataggg agatggaata cttgacaacc 9961 cttgagtacc taagggacga cagtgtggcg gtatcgtatt cactcaagga gaaggaggtg 10021 aaggttaatg ggcggatttt tgctaagttg acaaagagat taaggaactg ccaggtaatg 10081 gcagaaggga ttctagctga ccagattgca ccttttttcc agggaaatgg ggtcattcaa 10141 gatagcatat ccttgactaa gagtatgtta gcgatgagtc aactgtcttt taacagcaat 10201 aagaaacgta tcactgactg caaagagagg gtttcctcaa accgcaatca tgatccgaag 10261 agcaagaatc gtcgaagagt tgccactttt atcacgactg acctgcaaaa gtactgtctt 10321 aactggagat atcagacagt taagctattc gcccatgcta tcaatcagct gatgggtcta 10381 cctcacttct ttgagtggat tcaccttaga ctaatggata ctacaatgtt tgtaggagac 10441 cctttcaatc ctccaagtga cccgaccgac tgtgatctat caagagtccc aaatgatgat 10501 atatatattg tcagtgctag agggggcatc gagggattat gccagaagct atggacaatg 10561 atctcgattg ccgcaatcca acttgctgca gcaagatcgc attgtcgagt tgcctgcatg 10621 gtacaaggcg acaatcaagt aatagctgta acaagagaag taaggtcaga tgactcccca 10681 gatatggtat tgacacagtt gcatcaagct agtgataatt tcttcaaaga actgattcat 10741 gtcaatcatc tgattggcca caacctgaaa gatcgtgaaa ccatcagatc agacacattc 10801 ttcatataca gcaagcggat attcaaagac ggagcaatac tcagtcaggt cctcaaaaac 10861 tcatctaaat tggtactaat atcaggcgac cttagtgaaa acactgtaat gtcttgtgcc 10921 aacattgcat ccactgtagc acggctatgt gagaacgggc ttcctaagga tttttgttat 10981 tatttaaact acctaatgag ttgtgtgcag acgtactttg attctgaatt ttctattact 11041 cacagctcgc aaccagattc cacccagtct tggatcgagg acatctcttt tgtacactcg 11101 tatgttttaa cccctgccca gctaggagga ctgagtaacc ttcaatactc aaggctctac 11161 acaaggaata ttggtgaccc ggggactact gcttttgcag aggtcaagcg gttagaagca 11221 gtggggttgc tgagtcctag cattatgact aatatcttaa ctaggccacc tggcaatgga 11281 gactgggcca gcctgtgcaa cgatccttac tcctttaatt ttgagactgt cgcaagtcca 11341 aatatcgtcc ttaaaaagca cacacaaaaa gtcttatttg aaacttgttc aaacccttta 11401 ttatcaggag tacacacaga ggataatgag gcagaagaga aggcgctggc tgaattccta 11461 ctcaatcaag aagcggttca cccacgtgtc gcacatgcta tcatggaagc aagctctgta 11521 ggcaggagaa agcaaattca agggcttgtt gacactacaa acactgtgat taaaattgcg 11581 ctgaccagga gacccctcgg tatcaagagg ctgacacgga taatcaatta ctcgagcatg 11641 catgcaatgt tattcagaga tgatgttttc ttgcctaata gatccaacca cccattagtt 11701 tcgtctgcta tgtgttcgct gacgctagca gactatgctc ggaacagaag ctggtcacct 11761 ttaacagggg gtaggaaaat actgggtgta tctaaccccg ataccataga acttgtggag 11821 ggagagatcc ttagtgtcag tgggggatgc acaaaatgtg acagcggaga tgaacaattt 11881 acttggtttc atcttccaag caatatagag ctgactgatg acaccagcaa gaatcccccg 11941 atgagagtgc cgtaccttgg gtcaaagacc caagagagga gggctgcctc gctcgcgaaa 12001 atagctcata tgtcgccaca tgtgaaggca gcactaaggg catcatccgt attaatctgg 12061 gcttatgggg acaacgaagt aaattggact gctgctctta agattgcaag gtctcgatgt 12121 aacataagct cagagtatct tcggctactg tcacccctgc ccacagctgg gaatctccaa 12181 cataggttgg acgatggcat aacccagatg acatttaccc ctgcatctct ctacagagta 12241 tcaccttaca ttcacatatc caatgattcc caaagactat ttactgaaga aggaatcaaa 12301 gagggaaatg tggtttatca acaaattatg ctcctgggcc tatccctaat tgaatcactc 12361 ttcccaatga caacaaccaa gacatatgat gagattacat tacacctcca cagtaaattt 12421 agctgctgta tcagggaagc gcccgttgct gtccccttcg agttactcgg gttggcacca 12481 gaattaagga cggtaacctc aaataagttc atgtatgatc ctagccctat atcagagaga 12541 gattttgcga gacttgactt ggctatcttc aagagttacg aacttaattt agagtcatat 12601 tccacaatgg agctaatgaa cattctttca gtatctagtg ggaagttgat tggacagtcc 12661 gtggtttcct atgatgaaga tacctctata aagaatgatg ctataatagt gtatgataac 12721 acacgaaatt ggattagtga agcccagaac tcagatgtgg ttcgcctatt cgagtatgca 12781 gcgctcgaag tgctcctcga ttgttcttat caactctact atctgagagt gagaggtctc 12841 aacaatatcg tcttatacat gagtgactta tacaagaata tgccagggat tttactctct 12901 aatattgcgg ctacaatttc tcaccccatc attcactcaa ggttgaatgc agtaggtctg 12961 gtcaaccatg acgggtcaca tcagcttgcg gacacagatt tcattgaaat gtctgcgaag 13021 ttgttagtct cttgcactcg acgcgtggtc tcaggcttat atgcggggaa taagtatgat 13081 ctgctgttcc catctgtttt ggatgataac cttagtgaga agatgttgca gctgatttcc 13141 cggttatgct gtctgtatac agtacttttc gctacaacaa gagagatccc aaaaataagg 13201 gggctgtcag cggaagaaaa atgctcagta ctcactgagt acttactgtc agatgctgtg 13261 aaaccattgc ttaagtccga acaagtgagc tctattgtgt ctcccaacat aattacgttc 13321 ccagccaatc tatattacat gtctaggaag agccttaatt taatcagaga acgagaggac 13381 agagatgcca tcctgacgtt gttgttcccc caagaaccac tgttcgagct tcgtccagta 13441 caagacattg gtgctcgagt gagagatcct tttactcaac aacctgcatc attcatacaa 13501 gagctagatc taagtgctcc tgcaaggtat gacgcattta cccttagcga ggttcacttc 13561 gagcacacat tgccgaaccc aggagaggat tacttagttc ggtatttgtt cagaggaata 13621 gggactgctt catcttcttg gtataaggta tctcatcttc tttctgtccc tgaggtcaga 13681 tgtgcaagac atgggaactc cttatacttg gcggaaggaa gtggagccat catgagtatt 13741 cttgagctac atataccaca cgagaccatc tattacaata cgcttttctc gaatgagatg 13801 aaccctccac agcgacattt cggaccgacc cctacacagt ttctaaattc agttgtttat 13861 aggaatctac aggcggaagt gccatgcaaa gatggatttg tccaggagtt ctgcccatta 13921 tggagggaga atgcagaaga aagtgacttg acctcagata aggcagtggg atacatcaca 13981 tctgtggtgc cctataggtc tgtttcatta ctacattgtg atattgaaat tcctccaggg 14041 tctaatcaaa gcctgctaga tcaactggct actaatttat ctttgattgc catgcattct 14101 gtaagagagg gtggagtagt aatcatcaaa gtactgtatg taatgggata ctactttcat 14161 ctactcatga atttattcac tccgtgttcc acaaaaggat atattctctc caatggctat 14221 gcctgtagag gagatatgga gtgttacctg gtatttgtca tgggctattt gggcgggcct 14281 acattcgtaa acgaagtagt gaggatggca aaaactctaa tacaacagca cggtacactt 14341 ctgtctaaat cagatgagat tgcgttaacc aagctattta cctcacagca gcatcgtgta 14401 gtagacatcc tatccagccc tttaccgaga ctaatgaagt ttttgagaga aaatattgat 14461 gctgcactga ttgaagctgg gggacagccc gtccgtccat tctgtgcaga gagtttagtg 14521 ggcacattgg cagatatgac tcagatggct cagatcattg ctagccacat tgacacagtc 14581 attcgatctg tgatttacat ggaggctgag ggtgatcttg ctgacacagt gttcttgttt 14641 actccttaca atctctctac agacggtaag aagagaacat cactcaagca gtgcacaagg 14701 caaatcctag aggtcactat actgggtctc agagccagag atctcaacaa agtaggtgat 14761 gtaataggct taatactcag aggtacggtt tctctagagg acctcatccc attaaggaca 14821 tacctgaagc acagtacatg cccgaagtat ttgaaggcgg tcctagggat taccaaactt 14881 aaagaaatgt tcacagaaac ctctttacta tacctgactc gcgcccaaca aaaattctac 14941 atgaaaacca taggtaatgc agccaaggga tattacagca acaatgactc ctaaaggcaa 15001 ttatacatta atgaactatc tttctaatcg actgctccct cattgatttg ataataccgt 15061 attagaaaaa agttaaattc tgatcctttg aagttcgtac ccggattcag gcaattatct 15121 taaagcagaa gtgaacgtag ttatccttaa ttatagtcct gtcattcacc aaatctttg //