LOCUS NC_011048 19282 bp DNA linear PHG 26-MAR-2010 DEFINITION Bacillus phage phi29, complete genome. ACCESSION NC_011048 VERSION NC_011048.1 DBSOURCE BioProject accession PRJNA30615 KEYWORDS RefSeq. SOURCE Bacillus virus phi29 ORGANISM Bacillus virus phi29 Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae; Picovirinae; Phi29virus. REFERENCE 1 (bases 1 to 19282) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (11-JUL-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 2 (bases 1 to 19282) AUTHORS Villegas,A.P., Lingohr,E.J., Ceyssens,P.-J. and Kropinski,A.M. TITLE Direct Submission JOURNAL Submitted (29-MAY-2008) Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 110 Stone Road West, Guelph, Ontario N1G 3W4, Canada COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU771092. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..19282 /mol_type="genomic DNA" /db_xref="taxon:10756" /organism="Bacillus virus phi29" /host="Bacillus subtilis" repeat_region 1..6 /rpt_type=long_terminal_repeat /note="left terminal inverted repeat unit" gene complement(265..360) /locus_tag="phi29_gp0.2" /db_xref="GeneID:6446513" /gene="0.2" CDS complement(265..360) /locus_tag="phi29_gp0.2" /protein_id="YP_002004524.1" /gene="0.2" /transl_table=11 /note="similar to CAA24474.1 unnamed protein product Bacillus phage phi29" /db_xref="GeneID:6446513" /codon_start=1 /translation="MFDNYYRVCYNGSINGTVLPLSCVCWGLNPD" /product="hypothetical protein" regulatory complement(328..356) /regulatory_class="promoter" /locus_tag="phi29_gp0.2" /gene="0.2" /note="promoter A1; early transcription" gene complement(367..510) /locus_tag="phi29_gp0.4" /db_xref="GeneID:6446500" /gene="0.4" CDS complement(367..510) /locus_tag="phi29_gp0.4" /protein_id="YP_002004525.1" /gene="0.4" /transl_table=11 /note="similar to Bacillus phage PZA unknown protein product of ORF0 NP_040723" /db_xref="GeneID:6446500" /codon_start=1 /translation="MKRAELEEHIANMFKDGTIKLQVHPFGTNGVHLIVEINDQIVLE WEN" /product="hypothetical protein" gene complement(527..703) /locus_tag="phi29_gp0.6" /db_xref="GeneID:6446504" /gene="0.6" CDS complement(527..703) /locus_tag="phi29_gp0.6" /protein_id="YP_002004526.1" /gene="0.6" /transl_table=11 /note="similar to NP_040722.1 gene 1A product Bacillus phage PZA" /db_xref="GeneID:6446504" /codon_start=1 /translation="MSYLVTIFSINDNGVVRRYDTTARSRTDAASVLIKYKAPRGYKF DRFELSREKTIFDI" /product="hypothetical protein" gene complement(700..870) /locus_tag="phi29_gp0.8" /db_xref="GeneID:6446503" /gene="0.8" CDS complement(700..870) /locus_tag="phi29_gp0.8" /protein_id="YP_002004527.1" /gene="0.8" /transl_table=11 /note="similar to NP_040721.1 gene 1B product Bacillus phage PZA" /db_xref="GeneID:6446503" /codon_start=1 /translation="MVQNDFVDSYDVTMLLQDDDGKQYYEYHKGLSLSDFEVLYGNTA DEIIKLRLDKVL" /product="hypothetical protein" gene complement(857..1117) /locus_tag="phi29_gp1" /db_xref="GeneID:6446501" /gene="1" CDS complement(857..1117) /locus_tag="phi29_gp1" /function="involved in DNA replication" /protein_id="YP_002004528.1" /gene="1" /transl_table=11 /note="similar to NP_040720.1 gene 1C product Bacillus phage PZA; partially dispensible" /db_xref="GeneID:6446501" /codon_start=1 /translation="MGKIFDQEKRLEGTWKNSKWGNQGIIAPVDGDLKMIDLELEKKM TKLEHENKLMKNALYELSRMENNDYATWVIKVLFGGAPHGAK" /product="hypothetical protein" gene complement(1145..2863) /locus_tag="phi29_gp2" /db_xref="GeneID:6446511" /gene="2" CDS complement(1145..2863) /locus_tag="phi29_gp2" /protein_id="YP_002004529.1" /gene="2" /transl_table=11 /note="N-terminus contains 3'to 5' ssDNA exonuclease activity and C-terminus contains DNA polymerase activity; requires a protein (terminal protein) to prime DNA synthesis; belongs to the DNA polymerase type-B family." /db_xref="GeneID:6446511" /codon_start=1 /translation="MPRKMYSCDFETTTKVEDCRVWAYGYMNIEDHSEYKIGNSLDEF MAWVLKVQADLYFHNLKFDGAFIINWLERNGFKWSADGLPNTYNTIISRMGQWYMIDI CLGYKGKRKIHTVIYDSLKKLPFPVKKIAKDFKLTVLKGDIDYHKERPVGYKITPEEY AYIKNDIQIIAEALLIQFKQGLDRMTAGSDSLKGFKDIITTKKFKKVFPTLSLGLDKE VRYAYRGGFTWLNDRFKEKEIGEGMVFDVNSLYPAQMYSRLLPYGEPIVFEGKYVWDE DYPLHIQHIRCEFELKEGYIPTIQIKRSRFYKGNEYLKSSGGEIADLWLSNVDLELMK EHYDLYNVEYISGLKFKATTGLFKDFIDKWTYIKTTSEGAIKQLAKLMLNSLYGKFAS NPDVTGKVPYLKENGALGFRLGEEETKDPVYTPMGVFITAWARYTTITAAQACYDRII YCDTDSIHLTGTEIPDVIKDIVDPKKLGYWAHESTFKRAKYLRQKTYIQDIYMKEVDG KLVEGSPDDYTDIKFSVKCAGMTDKIKKEVTFENFKVGFSRKMKPKPVQVPGGVVLVD DTFTIK" /product="DNA polymerase" gene complement(2886..3686) /locus_tag="phi29_gp3" /db_xref="GeneID:6446514" /gene="3" CDS complement(2886..3686) /locus_tag="phi29_gp3" /protein_id="YP_002004530.1" /gene="3" /transl_table=11 /note="The terminal protein of phi29-like viruses is linked to the linear genome termini and serves as a primer for the initiation of DNA replication. Other names: gp3, TP" /db_xref="GeneID:6446514" /codon_start=1 /translation="MARSPRIRIKDNDKAEYARLVKNTKAKIARTKKKYGVDLTAEID IPDLDSFETRAQFNKWKEQASSFTNRANMRYQFEKNAYGVVASKAKIAEIERNTKEVQ RLVDEKIKAMKDKEYYAGGKPQGTIEQRIAMTSPAHVTGINRPHDFDFSKVRSYSRLR TLEESMEMRTDPQYYEKKMIQLQLNFIKSVEGSFNSFDAADELIEELKKIPPDDFYEL FLRISEISFEEFDSEGNTVENVEGNVYKILSYLEQYRRGDFDLSLKGF" /product="terminal protein" gene complement(3575..3952) /locus_tag="phi29_gp4" /db_xref="GeneID:6446523" /gene="4" CDS complement(3575..3952) /locus_tag="phi29_gp4" /protein_id="YP_002004531.1" /gene="4" /transl_table=11 /note="phi29 protein 4, together with protein 6, functions as a transcriptional activator for late promoter A3 and as a repressor for early promoters A2c and A2b. Therefore p4 and p6 regulate the switch from early to late expression. Other names: gp4, p4" /db_xref="GeneID:6446523" /codon_start=1 /translation="MPKTQRGIYHNLKESEYVASNTDVTFFFSSELYLNKFLDGYQEY RKKFNKKIERVAVTPWNMDMLADITFYSEVEKRGFHAWLKGDNATWREVHVYALRIMT KPNTLDWSRIQKPRLRERRKSMV" /product="transcriptional regulator" gene complement(3971..4345) /locus_tag="phi29_gp5" /db_xref="GeneID:6446522" /gene="5" CDS complement(3971..4345) /locus_tag="phi29_gp5" /protein_id="YP_002004532.1" /gene="5" /transl_table=11 /note="SSB; similar to NP_040716.1 gene 5A product Bacillus phage PZA, NP_690638.1 SSB Bacillus phage B103" /db_xref="GeneID:6446522" /codon_start=1 /translation="MENTNIVKATFDTETLEGQIKIFNAQTGGGQSFKNLPDGTIIEA NAIAQYKQVSDTYGDAKEETVTTIFAADGSLYSAISKTVAEAASDLIDLVTRHKLETF KVKVVQGTSSKGNVFFSLQLSL" /product="single stranded DNA-binding protein" gene complement(4410..4610) /locus_tag="phi29_gp5.5" /db_xref="GeneID:6446524" /gene="5.5" CDS complement(4410..4610) /locus_tag="phi29_gp5.5" /protein_id="YP_002004533.1" /gene="5.5" /transl_table=11 /note="similar to NP_040715.1 unknown protein Bacillus phage PZA" /db_xref="GeneID:6446524" /codon_start=1 /translation="MMETQEKVLVLVGALFINTFNLNYKSIDLTHDSEQAYGFKDKWE AQKVAANVGGQVVIRTTSFKIV" /product="hypothetical protein" gene complement(4607..4921) /locus_tag="phi29_gp6" /db_xref="GeneID:6446509" /gene="6" CDS complement(4607..4921) /locus_tag="phi29_gp6" /function="activates DNA replication; represses transcription from promoters C2, A2c and A2b; activates transcription fromn promoter A3" /protein_id="YP_002004534.1" /gene="6" /transl_table=11 /note="phi29 protein p6 together with protein p4 is involved in repression of transcription by early promoters A2c and A2b as well as activation of late promoter A3. In addition, p6 alone causes repression of early promoter C2. Thirdly, p6 appears to be involved in the initiation of DNA replication through binding to sites close to the termini of the genome. Other names: p6, DBP" /db_xref="GeneID:6446509" /codon_start=1 /translation="MAKMMQREITKTTVNVAKMVMVDGEVQVEQLPSETFVGNLTMEQ AQWRMKRKYKGEPVQVVSVEPNTEVYELPVEKFLEVATVRVEKDEDQEEQTEAPEEQV AE" /product="dsDNA binding protein" regulatory complement(4978..5006) /regulatory_class="promoter" /note="promoter A2c; early transcription" protein_bind 5095..5121 /bound_moiety="p4 protein" /note="activates transcription of late genes from A3 promoter; represses transcription fom early promoter A2c" gene 5222..5518 /locus_tag="phi29_gp7" /db_xref="GeneID:6446525" /gene="7" CDS 5222..5518 /locus_tag="phi29_gp7" /function="head morphogenesis" /protein_id="YP_002004535.1" /gene="7" /transl_table=11 /note="Other name: gp7; head morphogenesis protein" /db_xref="GeneID:6446525" /codon_start=1 /translation="MPLKPEEHEDILNKLLDPELAQSERTEALQQLRVNYGSFVSEYN DLTKSHEKLAAEKDDLIVSNSKLFRQIGLTDKQEEDHKKADISETITIEDLEAK" /product="scaffolding protein" gene 5549..6895 /locus_tag="phi29_gp8" /db_xref="GeneID:6446510" /gene="8" CDS 5549..6895 /locus_tag="phi29_gp8" /protein_id="YP_002004536.1" /gene="8" /transl_table=11 /note="Other names: p8" /db_xref="GeneID:6446510" /codon_start=1 /translation="MRITFNDVKTSLGITESYDIVNAIRNSQGDNFKSYVPLATANNV AEVGAGILINQTVQNDFITSLVDRIGLVVIRQVSLNNPLKKFKKGQIPLGRTIEEIYT DITKEKQYDAEEAEQKVFEREMPNVKTLFHERNRQGFYHQTIQDDSLKTAFVSWGNFE SFVSSIINAIYNSAEVDEYEYMKLLVDNYYSKGLFTTVKIDEPTSSTGALTEFVKKMR ATARKLTLPQGSRDWNSMAVRTRSYMEDLHLIIDADLEAELDVDVLAKAFNMNRTDFL GNVTVIDGFASTGLEAVLVDKDWFMVYDNLHKMETVRNPRGLYWNYYYHVWQTLSVSR FANAVAFVSGDVPAVTQVIVSPNIAAVKQGGQQQFTAYVRATDAKDHKVVWSVEGGST GTAITGDGLLSVSGNEDNQLTVKATVDIGTEDKPKLVVGEAVVSIRPNNASGGAQA" /product="major head protein" gene 6895..7737 /locus_tag="phi29_gp8.5" /db_xref="GeneID:6446512" /gene="8.5" CDS 6895..7737 /locus_tag="phi29_gp8.5" /protein_id="YP_002004537.1" /gene="8.5" /transl_table=11 /note="similar to NP_040726.1 head fiber protein Bacillus phage PZA" /db_xref="GeneID:6446512" /codon_start=1 /translation="MMVSFTARAKSNVMAYRLLAYSQGDDIIEISHAAENTIPDYVAV KDVDKGDLTQVNMYPLAAWQVIAGSDIKVGDNLTTGKDGTAVPTDDPSTVFGYAVEEA QEGQLVTLVISRSKEISIEVDDIKDAGDTGKRLLKINTPSGARNIIIENEDAKALING ETTNTNKKNLQDLLFSDGNVKAFLQATTTDENKTALQQLLVSNADVLGLLSGNPTSDN KINLRTMIGAGVPYSLPAATTTTLGGVKKGAAVTASTATDVATAVKDLNSLITVLKNA GIIS" /product="head fiber protein" gene 7751..9550 /locus_tag="phi29_gp9" /db_xref="GeneID:6446507" /gene="9" CDS 7751..9550 /locus_tag="phi29_gp9" /protein_id="YP_002004538.1" /gene="9" /transl_table=11 /note="Other names: p9" /db_xref="GeneID:6446507" /codon_start=1 /translation="MAYVPLSGTNVRILADVPFSNDYKNTRWFTSSSNQYNWFNSKSR VYEMSKVTFMGFRENKPYVSVSLPIDKLYSASYIMFQNADYGNKWFYAFVTELEFKNS AVTYVHFEIDVLQTWMFDIKFQESFIVREHVKLWNDDGTPTINTIDEGLSYGSEYDIV SVENHKPYDDMMFLVIISKSIMHGTPGEEESRLNDINASLNGMPQPLCYYIHPFYKDG KVPKTYIGDNNANLSPIVNMLTNIFSQKSAVNDIVNMYVTDYIGLKLDYKNGDKELKL DKDMFEQAGIADDKHGNVDTIFVKKIPDYEALEIDTGDKWGGFTKDQESKLMMYPYCV TEITDFKGNHMNLKTEYINNSKLKIQVRGSLGVSNKVAYSVQDYNADSALSGGNRLTA SLDSSLINNNPNDIAILNDYLSAYLQGNKNSLENQKSSILFNGIMGMIGGGISAGASA AGGSALGMASSVTGMTSTAGNAVLQMQAMQAKQADIANIPPQLTKMGGNTAFDYGNGY RGVYVIKKQLKAEYRRSLSSFFHKYGYKINRVKKPNLRTRKAFNYVQTKDCFISGDIN NNDLQEIRTIFDNGITLWHTDNIGNYSVENELR" /product="tail protein" gene 9555..10484 /locus_tag="phi29_gp10" /db_xref="GeneID:6446518" /gene="10" CDS 9555..10484 /locus_tag="phi29_gp10" /protein_id="YP_002004539.1" /gene="10" /transl_table=11 /note="In phage phi29, 12 copies of the head-tail connector/upper collar protein are a component of the DNA packaging motor that imports DNA into the empty pro-head. Other names: head-tail connector; p10" /db_xref="GeneID:6446518" /codon_start=1 /translation="MARKRSNTYRSINEIQRQKRNRWFIHYLNYLQSLAYQLFEWENL PPTINPSFLEKSIHQFGYVGFYKDPVISYIACNGALSGQRDVYNQATVFRAASPVYQK EFKLYNYRDMKEEDMGVVIYNNDMAFPTTPTLELFAAELAELKEIISVNQNAQKTPVL IRANDNNQLSLKQVYNQYEGNAPVIFAHEALDSDSIEVFKTDAPYVVDKLNAQKNAVW NEMMTFLGIKNANLEKKERMVTDEVSSNDEQIESSGTVFLKSREEACEKINELYGLNV KVKFRYDIVEQMRRELQQIENVSRGTSDGETNE" /product="upper collar protein" gene 10477..11358 /locus_tag="phi29_gp11" /db_xref="GeneID:6446508" /gene="11" CDS 10477..11358 /locus_tag="phi29_gp11" /protein_id="YP_002004540.1" /gene="11" /transl_table=11 /note="Other names: p11" /db_xref="GeneID:6446508" /codon_start=1 /translation="MSSYTMQLRTYIEMWSQGETGLSTAEKIEKGRPKLFDFNYPIFD ESYRTIFETHFIRNFYMREIGFETEGLFKFHLETWLMINMPYFNKLFESELIKYDPLE NTRVGVKSNTKNDTDRNDNRDVKQDLTSNGTSSTDAKQNDTSKTTGNEKSSGSGSITD DNFKRDLNADTADDRLQLTTKDGEGVLEYASQIEEHNENKKRDTKTSNTTDTTSNTTG TSTLDSDSKTSNKANTTSNDKLNSQINSVEDYIEDRVGKIGTQSYARLVMDYREALLR IEQRIFNEMQELFMLVY" /product="lower collar protein" gene 11371..13935 /locus_tag="phi29_gp12" /db_xref="GeneID:6446499" /gene="12" CDS 11371..13935 /locus_tag="phi29_gp12" /protein_id="YP_002004541.1" /gene="12" /transl_table=11 /note="similar to NP_040730.1 pre-neck appendage protein Bacillus phage PZA, NP_690646.1 pre-neck appendage protein Bacillus phage B103" /db_xref="GeneID:6446499" /codon_start=1 /translation="MSTKPELKRFEQFGEMMVQLYERYLPTAFDESLTLLEKMNKIIH YLNEIGKVTNELIEEWNKVMEWILNDGLEDLVKETLERWYEEGKFADLVIQVIDELKQ FGVSVKTYGAKGDGVTDDIRAFEKAIESGFPVYVPYGTFMVSRGIKLPSNTVLTGAGK RNAVIKFMDSVGRGESLMYNENVTTGNENIFLSSFTLDGNNKRLGQGISGIGGSRESN LSIRACHNVYIRDIEAVDCTLHGIDITCGGLDYPYLGDGTTAPNPSENIWIENCEATG FGDDGITTHHSQYINILNCYSHDPRLTANCNGFEIDDGSRHVVLSNNRSKGCYGGIEI KAHGDAPAAYNISINGHMSVEDVRSYNFRHIGHHAATAPQSVSAKNIVASNLVSIRPN NKRGFQDNATPRVLAVSAYYGVVINGLTGYTDDPNLLTETVVSVQFRARNCSLNGVVL TGFSNSENGIYVIGGSRGGDAVNISNVTLNNSGRYGVSIGSGIENVSITNISGIGDGI NSPVALVSTINSNPEISGLSSIGYPTVARVAGTDYNDGLTLFNGAFRASTTSSGKIHS EGFIMGSTSGCEASVSKSGVLTSSSSKTSSERSLIAGSSTSEAKGTYNTILGSLGAVA DEKFAALISASQSRASGNHNLILSSYGINTTGSYKVNGGFEKINWELDSLNGRIKARD TVTGGNTWSDFAEYFESLDGQVIETGYLVTLEKGKIRKAEKGEKIIGVISETAGFVLG ESSFEWQGAVLKNEFGGIIYEEVTTEDGVKFKRPLPNPDFDPNKNYIPRSQRREWHVV GLLGQIAVRIDETVKQGHGIDAVGGVATDGDNFIVQEITTPYTKEKGYGVAIVLVK" /product="preneck appendage protein" gene 13947..15041 /locus_tag="phi29_gp13" /db_xref="GeneID:6446506" /gene="13" CDS 13947..15041 /locus_tag="phi29_gp13" /function="tail assembly" /protein_id="YP_002004542.1" /gene="13" /transl_table=11 /note="similar to NP_040731.1 morphogenesis protein A Bacillus phage PZA, NP_690647.1 morphogenesis protein Bacillus phage B103" /db_xref="GeneID:6446506" /codon_start=1 /translation="MVYVSNKYLTMSEMKVNAQYILNYLSSNGWTKQAICGMLGNMQS ESTINPGLWQNLDEGNTSLGFGLVQWTPASNYINWANSQGLPYKNMDSELKRIIWEVN NNAQWINLRDMTFKEYIKSTKTPRELAMIFLASYERPANPNQPERGDQAEYWYKNLSG GGGGGLQLAQFPMDIINISQGENGSFSHKGTLCIDFVGKTEKYPYYAPCDCTCVWRGD ASAYLAWTSDKEVMCADGSVRYITWVNVHESPLPFDVGKKLKKGDLMGHTGIGGNVTG DHWHFNVIDGKEYQGWTKKPDSCLAGTELHIYDVFAVNNVEIINGNGYDWKTSDWQDG DGGDGDDNDNNKTKDLITLLLSDALHGWKA" /product="morphogenesis protein" gene 15060..15455 /locus_tag="phi29_gp14" /db_xref="GeneID:6446519" /gene="14" CDS 15060..15455 /locus_tag="phi29_gp14" /function="lysis protein" /protein_id="YP_002004543.1" /gene="14" /transl_table=11 /note="similar to NP_040732.1 lysis protein Bacillus phage PZA; putative dual start motif" /db_xref="GeneID:6446519" /codon_start=1 /translation="MKMIAWMQHFLETDETKLIYWLTFLMVCMVVDTVLGVLFAKLNP NIKFSSFKIKTGVLIKVSEMILALLAIPFAVPFPAGLPLLYTVYTALCVSEIYSIFGH LRLVDDKSDFLEILENFFKRTSGKNKEEK" /product="holin" gene 15457..16233 /locus_tag="phi29_gp15" /db_xref="GeneID:6446520" /gene="15" CDS 15457..16233 /locus_tag="phi29_gp15" /function="lysis protein" /protein_id="YP_002004544.1" /gene="15" /transl_table=11 /note="similar to NP_040733.1 morphogenesis protein B Bacillus phage PZA, AAK40280.1 endolysin Bacillus amyloliquefaciens phage Morita2001, NP_690649.1 morphogenesis protein Bacillus phage B103; pfam00959, phage_lysozyme" /db_xref="GeneID:6446520" /codon_start=1 /translation="MQISQAGINLIKSFEGLQLKAYKAVPTEKHYTIGYGHYGSDVSP RQVITAKQAEDMLRDDVQAFVDGVNKALKVSVTQNQFDALVSFAYNVGLGAFRSSSLL EYLNEGRTALAAAEFPKWNKSGGKVYQGLINRRAQEQALFNSGTPKNVSRGTSSTKTT PKYKVKSGDNLTKIAKKHNTTVATLLKLNPSIKDPNMIRVGQTINVTGSGGKTHKVKS GDTLSKIAVDNKTTVSRLMSLNPEITNPNHIKVGQTIRLS" /product="peptidoglycan hydrolase" gene 16244..17242 /locus_tag="phi29_gp16" /db_xref="GeneID:6446516" /gene="16" CDS 16244..17242 /locus_tag="phi29_gp16" /protein_id="YP_002004545.1" /gene="16" /transl_table=11 /note="the phi 29 protein p16 is a DNA packaging ATPase. Several molecules of p16 along with a hexamer of packaging RNA (pRNA) and 12 molecules of the head-to-tail connector form a packaging motor. Packaging consumes about 1 ATP per 2 bp and can operate against strong loads." /db_xref="GeneID:6446516" /codon_start=1 /translation="MDKSLFYNPQKMLSYDRILNFVIGARGIGKSYAMKVYPINRFIK YGEQFIYVRRYKPELAKVSNYFNDVAQEFPDHELVVKGRRFYIDGKLAGWAIPLSVWQ SEKSNAYPNVSTIVFDEFIREKDNSNYIPNEVSALLNLMDTVFRNRERVRCICLSNAV SVVNPYFLFFNLVPDVNKRFNVYDDALIEIPDSLDFSSERRKTRFGRLIDGTEYGEMS LDNQFIGDSQVFIEKRSKDSKFVFSIVYNGFTLGVWVDVNQGLMYIDTAHDPSTKNVY TLTTDDLNENMMLITNYKNNYHLRKLASAFMNGYLRFDNQVIRNIAYELFRKMRIQ" /product="DNA encapsidation protein" regulatory complement(17262..17295) /regulatory_class="terminator" /note="Rho-independent terminator" regulatory 17271..17301 /regulatory_class="terminator" /note="Rho-independent terminator" gene complement(17383..17547) /locus_tag="phi29_gp16.6" /db_xref="GeneID:6446505" /gene="16.6" CDS complement(17383..17547) /locus_tag="phi29_gp16.6" /protein_id="YP_002004546.1" /gene="16.6" /transl_table=11 /note="similar to NP_040736.1 gene 16.6 product Bacillus phage PZA" /db_xref="GeneID:6446505" /codon_start=1 /translation="MKLLTHVCHYCSFSFFTRKFDVFGAITKKDTPVVFCPTCGNQSL SVSHIEEEIR" /product="hypothetical early protein" gene complement(17544..17936) /locus_tag="phi29_gp16.7" /db_xref="GeneID:6446517" /gene="16.7" CDS complement(17544..17936) /locus_tag="phi29_gp16.7" /protein_id="YP_002004547.1" /gene="16.7" /transl_table=11 /note="similar to NP_040737.1 putative replication organizer protein P16.7 Bacillus phage PZA" /db_xref="GeneID:6446517" /codon_start=1 /translation="MEAILMIGVLALCVIFLLSGRNNKKKQEARELEDYLEDLNKRVV QRTQILSELNEVISNRSIDKTVNLSACEVAVLDLYEQSNIRIPSDIIEDLVNQRLQSE QEVLNYIETQRTYWKLENQKKLYRGSLK" /product="hypothetical early protein" gene complement(17938..18255) /locus_tag="phi29_gp16.8" /db_xref="GeneID:6446502" /gene="16.8" CDS complement(17938..18255) /locus_tag="phi29_gp16.8" /protein_id="YP_002004548.1" /gene="16.8" /transl_table=11 /note="similar to NP_040738.1 unknown protein Bacillus phage PZA" /db_xref="GeneID:6446502" /codon_start=1 /translation="MIDIIVKEDKRLITVQTPEGDEVFYTLSFSDGHKILKRSSARLR NNIYAIGVANIRWMLVDMDNMILSEYIHHVDILKDIDRKMREMGYIVISEWQHANKKG TRR" /product="hypothetical early protein" gene complement(18252..18578) /locus_tag="phi29_gp16.9" /db_xref="GeneID:6446515" /gene="16.9" CDS complement(18252..18578) /locus_tag="phi29_gp16.9" /protein_id="YP_002004549.1" /gene="16.9" /transl_table=11 /note="similar to NP_040739.1 gene 16.9 product Bacillus phage PZA" /db_xref="GeneID:6446515" /codon_start=1 /translation="MSVQLNAFTFILERRGWRMVCYEQLTTNGTRILHFYLKDNPTFF ATYSSQFLSDTKMIRRFASWSGQLLEGSNSVFWTNITPFEPIDEETAEDIKNLDKVVE GMNFTL" /product="hypothetical early protein" gene complement(18580..19080) /locus_tag="phi29_gp17" /db_xref="GeneID:6446521" /gene="17" CDS complement(18580..19080) /locus_tag="phi29_gp17" /protein_id="YP_002004550.1" /gene="17" /transl_table=11 /note="similar to NP_040740.1 gene 17 product Bacillus phage PZA, VG17_BPPH5 early protein GP17 Bacillus phage phi15" /db_xref="GeneID:6446521" /codon_start=1 /translation="MNNYQLTINEVIDIINTNTEINKLVAKKENLFPTDLYDLDKQEL IAIILNSDFALSSIKRVLLEVTVEELGTQDNDEDDELEDLDGEIDRVDYIDKDGIRFD VPRETSPHVDKSIVTFNDELLDEANKIAKSIQEHDFNDKAIEEAELKIFKNHLPSIYS MKKENK" /product="hypothetical early protein" regulatory complement(19124..19158) /regulatory_class="promoter" /note="early promoter C2; activity repressed by protein p6" repeat_region complement(19277..19282) /rpt_type=long_terminal_repeat /note="right terminal inverted repeat unit" ORIGIN 1 aaagtaagcc cccaccctca catgatacca ttctcctaat atcgacataa tccgtcgatc 61 ctcggcatac catgatcagg gagggaaact actacttaat atatcaatct atagacctac 121 tagataggtt tgtcaatgaa caacataaaa cgacacagaa tcccacgttt tagcgcttcg 181 tctgtgtcgc atgtaaaagg ttatcaaagt agcgtgcact tttgccatga ttgacaacca 241 atcaacaaag tatgtgggct gaactcaatc agggtttaat ccccaacata cacatgacaa 301 tggaagtacc gtaccattga tactaccatt atagcatact ctgtaatagt tgtcaaacat 361 taatagttaa ttctcccatt ctaaaacgat ttgatcgttt atttctacaa ttagatggac 421 tccatttgta ccgaatggat ggacttgtaa ctttatcgta ccatctttaa acatattcgc 481 aatatgttct tctaactctg cacgcttcat gatataaccc tccttatcag atgtcaaata 541 tagttttctc acggctcaac tcaaacctat caaacttgta acccctcggc gctttatact 601 taatcaacac actagccgca tccgttctac tacgtgctgt cgtatcatag cgcctaacta 661 ccccattgtc gttaatagag aagatagtta ctagataact catagtactt tatccaacct 721 tagttttata atttcatcag cagtgttacc gtatagaacc tcaaagtctg acaaactcag 781 tcccttgtgg tactcataat actgtttacc gtcatcatct tgaagcaaca ttgtcacatc 841 gtatgagtca acaaaatcat tttgcaccat gtggagcacc tccaaataac acctttataa 901 cccatgtggc gtaatcattg ttttccatcc tagaaagctc atacaatgcg tttttcatga 961 gtttattttc atgctctagt ttagtcatct tcttttctaa ctcaaggtct atcatcttta 1021 agtctccgtc tacaggtgct ataatgcctt gattacccca tttagagttt ttccatgtgc 1081 cttctaatct cttctcttga tcgaagattt tacccattac aaacatctcc ttttaaacaa 1141 acgtttattt gattgtgaat gtgtcatcaa ccagaaccac cccgcccggc acttgcacag 1201 gcttaggctt cattttccga ctgaatccga ctttgaaatt ctcaaacgta acctctttct 1261 taatcttgtc agtcattccc gcacatttaa cactaaattt tatatcagtg taatcatctg 1321 gactaccttc tactaactta ccatctactt ctttcatata gatgtcttgt atataggtct 1381 tctgtctcag atatttagct cttttgaatg tactttcatg tgcccagtat cccaatttct 1441 tagggtcaac tatatctttt attacatcag gtatctctgt acccgttaaa tgtatgctgt 1501 cagtatcaca gtatattatc cgatcataac aagcctgtgc cgctgtaatt gtcgtgtatc 1561 tagcccatgc agtgatgaaa acgcccatag gtgtataaac agggtctttt gtttcctctt 1621 ctccaagtct gaaacctagc gccccattct cttttaaata agggactttc cctgtaacat 1681 cagggttact agcgaattta ccgtatagac tgtttaacat cagttttgct agttgcttga 1741 tcgctccttc tgatgtcgtc ttgatgtacg tccatttatc tataaaatct ttaaacaaac 1801 ctgtagttgc tttaaatttt aagccgctga tatattcaac gttatataaa tcgtagtgtt 1861 ctttcattaa ttctaggtct acatttgaca accagaggtc ggctatctcc ccgccgctac 1921 tttttaggta ctcattacct ttataaaacc tacttctttt tatctgtata gtgggtatat 1981 agccctcttt caattcgaac tcacatctga tatgctgtat gtgtagtggg taatcttcgt 2041 cccaaacgta tttaccctcg aatactatag gttcaccata tggaaggaga cggctataca 2101 tctgtgcagg atatagacta ttaacatcga agaccatgcc ttctccgatt tctttttctt 2161 tgaacctatc atttaaccat gtaaaaccac ctctataggc gtatctcact tccttatcga 2221 gtccaagact caatgtagga aacacctttt tgaatttctt agtggttata atatccttga 2281 aaccttttag actgtcactg cctgctgtca tccggtctaa accttgctta aactgaatta 2341 acagagcttc cgcaataatc tgaatatcgt ttttaatata ggcgtattct tcgggtgtta 2401 tcttatagcc gactggtctt tctttgtggt aatcaatatc acctttaaga acagttagtt 2461 taaagtcttt agctatcttc ttaacaggaa acggtagttt ctttaagctg tcatatatca 2521 ctgtatgtat cttacgtttc cctttgtagc ctaaacatat atcaatcatg taccattgtc 2581 ccatgcgaga tatgatcgta ttatatgtgt ttggcaatcc gtcagccgac cacttaaaac 2641 cattacgttc caaccagtta atgataaaag ctccgtcaaa tttgaggtta tggaaatata 2701 gatcagcttg taccttcaac acccacgcca taaactcatc caggctatta cctattttgt 2761 actcactgtg atcttctata ttcatataac catacgccca taccctacag tcttccactt 2821 tagtagttgt ctcaaagtca caactataca tctttctcgg catatgcttc atcctttaac 2881 ggagcctaga acccctttaa gcttagatca aagtcacccc ttcgatactg ttccaagtat 2941 gacagtattt tatatacatt accttctacg ttctccactg tgtttccctc actatcaaat 3001 tcctcaaagg atatttctga tattctgaga aacaattcat agaagtcatc aggaggtatc 3061 ttttttaatt cttcgatcag ttcatctgcc gcatcaaatg aattgaaact accctcaacg 3121 ctcttaataa agtttaactg taactgtatc attttctttt cataatactg agggtctgtt 3181 ctcatctcca tgctttcttc tagggttcgc aaacggctat agcttcgcac cttgctaaag 3241 tcaaaatcat ggggtctatt aattcctgta acgtgtgcag gacttgtcat agctatccgt 3301 tgttcaattg tcccttgcgg cttaccgcct gcatagtatt ctttgtcttt catagccttg 3361 attttctcat ctactaaccg ctgaacctct tttgtgttac gttcaatctc agctatctta 3421 gctttactag ccaccacacc gtatgcattc ttttcgaact gataacgcat attagcacgg 3481 ttagtgaaag aggacgcttg ttccttccac ttattgaact gcgcccgtgt ttcaaatgaa 3541 tcaaggtcag gtatatcaat ttcagcggta aggtctacac catacttttt cttcgttctc 3601 gcaatcttgg cttttgtatt cttgaccaat cgagcgtatt cggctttgtc attatcctta 3661 atgcgtatac gtggacttct cgccatgttg cgttatctcc tttcaaccaa gcatggaaac 3721 cacgcttttc aacttctgaa tagaacgtga tgtctgcgag catatccata ttccacggtg 3781 taacagcgac ccgttctatc ttcttattaa atttcttcct gtattcttgg tatccatcga 3841 gaaacttgtt caaatacaat tcacttgaaa agaaaaacgt gacatcggtg ttagatgcca 3901 cgtattcaga ttccttcaag ttatgataga tacctctttg tgttttaggc atctatattt 3961 tacctcctgt ttatagggat agttgtaagc taaagaatac gttaccttta ctagatgttc 4021 cttgaaccac tttaacctta aacgtttcaa gcttatgacg agtcacaagg tcaattaagt 4081 cagatgcggc ttctgctaca gtcttagaga tagcggaata taacgacccg tcagccgcaa 4141 aaatagtagt aactgtttct tccttagcgt ccccgtatgt atcggacact tgcttatatt 4201 gagcaatggc gttggcttct ataattgttc catctggaag gtttttaaaa gattgtccgc 4261 cgcctgtctg agcattaaag attttgattt gtccttcaag agtttctgtg tcaaaagtag 4321 cctttacgat gtttgtgttt tccattatta atcatccttt tcattttggt ttatttgata 4381 ggtcgtgcgt gttaagagag tgttaagagt taaacgatct tgaaactggt tgttctgatg 4441 acaacttgtc cgcctacatt tgcggcaact ttctgcgctt cccatttatc tttaaacccg 4501 taagcttgtt ctgaatcgtg tgttaggtca atgcttttat aattaagatt gaaggtgttt 4561 atgaatagcg ctccaactaa tactagaacc ttctcttgtg tttccatcat tcagcaacct 4621 gttcttctgg agcttctgtt tgttcctctt gatcttcgtc tttctctacc cgaacggtag 4681 caacttcaag gaatttttct acaggtagct cataaacctc tgtgttaggt tcaacgctta 4741 ccacttgaac aggttcgcct ttatatttgc gcttcattct ccattgagcc tgttccattg 4801 tcagattacc cacaaatgtt tcagatggta gttgctctac ctgaacctct ccgtccacca 4861 tcaccatttt ggcaacgttg acggttgtct ttgtgatttc tctctgcatc atttttgcca 4921 tttcttcgtc ccactttcta tttatatttg tagactctgt atctcttcta ctcttttatt 4981 atatcaccct gttcgacaat tttcaacact tttctataaa aagttcgaca aagttttaca 5041 aggttttaat aaaaggttgt tgtctacgtt ctctatttaa ccacacctgt ataacttttt 5101 gcaagacttt tttataaaat gttgacgttt ttcgacaaga acgcccacaa atccttatgt 5161 atcaagggtt cacgtggtat aattaagtag tactaataga ttatagaaag aggtgaaaca 5221 tatgccatta aaaccagaag aacacgaaga tattttgaat aagcttttag accctgagtt 5281 ggctcaatca gaaagaacgg aagctcttca acagctcaga gtgaactacg gttcttttgt 5341 gtccgagtac aatgatttaa caaaatctca tgaaaagtta gccgctgaaa aagatgatct 5401 gatcgtgtca aatagtaagc ttttcagaca gattggtttg acagacaaac aggaagaaga 5461 tcacaagaaa gctgatatta gtgaaacaat cacgatcgag gacttagaag caaagtaatt 5521 ataatagaag aaaagaggta caacattcat gcgaattaca tttaacgacg tgaaaacgtc 5581 tctaggtatt accgaatcat acgacatcgt gaatgccatt cgtaacagtc agggcgataa 5641 cttcaaaagt tatgtgccgc tagcaactgc gaacaacgtc gcagaagtcg gggcaggtat 5701 cctgatcaat caaacggtac aaaatgattt catcacgtcc cttgttgatc gtattggact 5761 tgtggttatc cgacaggtgt cgttaaacaa cccgttgaag aaattcaaga aaggtcaaat 5821 tcctctcggt cgtactatcg aagagattta tacagacatc acaaaagaaa aacagtacga 5881 tgctgaggaa gccgaacaga aggtatttga acgtgaaatg ccgaacgtca aaactctctt 5941 ccacgagaga aacagacagg gcttttatca tcaaacaatc caagatgact cactcaaaac 6001 tgcatttgta tcatggggta atttcgaaag ctttgtttct tctattatta atgctatcta 6061 caatagtgct gaagtagacg aatacgaata tatgaaattg ttagttgaca actactattc 6121 aaaagggttg ttcactactg taaaaatcga tgagccgact tcttctacag gggcattaac 6181 tgaatttgtg aagaaaatgc gtgcgactgc tcgtaaactc acattgccgc aaggttcccg 6241 tgactggaac tcaatggctg ttcgaacacg ctcttatatg gaagatttac acctaatcat 6301 tgacgctgac cttgaagctg aactagatgt tgatgtttta gctaaggcat tcaatatgaa 6361 ccgtactgac ttcctcggaa atgtgacggt cattgatggt ttcgcatcca ctggactaga 6421 agctgtttta gttgacaaag actggtttat ggtgtatgac aacctccata aaatggagac 6481 ggttcgtaac ccacgtggtc tgtactggaa ctattactac catgtgtggc agaccctaag 6541 cgtgtcccgt tttgccaatg ccgttgcgtt tgtatctggt gatgttcctg ctgttactca 6601 ggttattgtt tctcctaaca tcgccgcagt taaacaaggc ggacaacaac aattcactgc 6661 ctatgttcgt gctacagacg ctaaagatca taaagtcgtt tggagcgttg aaggtggttc 6721 aacgggtaca gctattacag gggatgggct tctttctgtc agcggaaacg aagataatca 6781 gttaacagta aaagcaactg ttgacatcgg aacagaagac aaaccaaaac tcgttgtggg 6841 tgaagctgtt gtatctattc gtccgaacaa tgcctctgga ggtgctcagg cgtaatgatg 6901 gtttcattta ctgcacgtgc aaagtctaat gttatggcat atcgtttact agcgtattca 6961 cagggagacg acatcataga aatttcacac gccgcagaaa atactatacc tgattacgtt 7021 gccgttaaag acgttgacaa gggtgatcta acacaagtca acatgtaccc tttagccgct 7081 tggcaagtta tcgccggaag tgacatcaaa gtgggcgata atctcacaac tggtaaggat 7141 ggtactgctg ttccaaccga cgaccctagc actgtttttg ggtacgctgt cgaagaggct 7201 caggaaggac agctagtcac cctcgttatc agccgatcaa aagaaatttc tatagaagta 7261 gatgatatta aggacgccgg agacactggg aaacggttat tgaaaatcaa taccccgtcc 7321 ggtgctcgta atatcattat tgaaaatgaa gacgctaaag cattgataaa tggtgaaacc 7381 accaacacaa ataagaagaa ccttcaagac ctactatttt cagacggtaa tgttaaggct 7441 tttctacaag ctaccacgac cgatgagaat aaaacggctc ttcaacaatt gttggttagc 7501 aacgccgatg tgcttggtct tctgtcagga aacccaacat ctgacaataa aattaacctt 7561 cgcacaatga tcggggcggg cgttccatac tcgctccccg ccgctacaac gacaacgtta 7621 ggcggtgtga agaaaggtgc ggcagttaca gcatctacag caaccgacgt ggcgactgcg 7681 gttaaagact tgaacagttt aattactgtt ttaaagaacg caggaatcat atcataaggt 7741 ggtttgatta atggcatatg taccattatc aggaacgaac gtcaggattt tagctgacgt 7801 tcctttctct aatgattata aaaacacgag atggttcaca tcttcaagta atcagtataa 7861 ctggtttaac agcaaatcac gtgtgtatga aatgagtaaa gtaacattca tggggtttag 7921 agaaaataaa ccatatgttt cggttagtct tcccatagat aagctttaca gtgcgtcata 7981 tattatgttt caaaatgcag actacggtaa caagtggttt tatgcatttg taaccgagtt 8041 agaatttaaa aatagtgctg ttacctacgt tcactttgaa attgatgttc tccaaacatg 8101 gatgttcgat attaaatttc aagaatcatt cattgtgagg gagcacgtta aattatggaa 8161 tgacgacggg acaccgacta tcaacacaat tgatgagggt ctcagctacg gaagtgaata 8221 cgacatagtt tctgtagaaa accataaacc atacgacgac atgatgtttc tcgtgattat 8281 ttccaaaagc attatgcatg ggacgccggg agaagaggaa agcaggctaa atgacataaa 8341 cgcaagcctg aacggcatgc cgcaacctct ctgctactat attcacccat tctacaaaga 8401 tggtaaagtt cctaaaacgt atatcggaga taacaacgct aacttgtctc ctattgtcaa 8461 tatgctcacc aatatctttt cacagaagag cgctgttaac gatattgtca atatgtatgt 8521 gactgattat attggtttga agcttgacta taaaaatggt gataaagaat tgaagctcga 8581 taaagacatg tttgaacagg cgggtatagc tgacgataaa cacggtaacg ttgacaccat 8641 ctttgtgaag aaaatacctg attatgaagc cctagaaata gacacaggtg ataaatgggg 8701 tggcttcaca aaagaccaag aaagcaaact gatgatgtac ccttactgcg ttacggaaat 8761 aactgacttt aaaggcaacc atatgaatct gaaaaccgag tacatcaata acagtaaact 8821 aaagatacag gttaggggtt cactaggggt cagtaacaag gttgcctaca gtgttcagga 8881 ttataacgca gatagcgcat tgagtggcgg caatagattg actgcgtctc tagattcatc 8941 cttaatcaac aacaacccaa atgacatagc aatactaaat gactatctat ctgcttattt 9001 acagggcaac aaaaattcac tagagaacca aaaatcgtct atccttttta atggcattat 9061 gggtatgatc ggcggaggta tatcagcggg agcaagtgcg gcaggaggtt cagccctagg 9121 gatggcttca tcagttacag ggatgacaag cactgcgggt aatgctgttc tacagatgca 9181 agcgatgcaa gccaagcaag ccgatatagc aaacattccg ccgcagttaa cgaaaatggg 9241 cggcaacaca gcgtttgatt acgggaatgg gtacagaggt gtgtacgtca tcaaaaagca 9301 attgaaggct gaatacagac gaagtctatc aagtttcttc cataaatacg gatacaagat 9361 taacagggta aagaaaccaa atttaagaac acgaaaagca tttaactatg ttcagacaaa 9421 agactgtttc atttcagggg acatcaataa caatgactta caggaaataa gaacaatttt 9481 cgataatggt attactcttt ggcatactga caacatcgga aattacagcg tcgagaatga 9541 attgaggtga cacaatggca cgtaaacgca gtaacacata ccgatctatc aatgagatac 9601 agcgtcaaaa acggaataga tggtttattc actatctgaa ctaccttcaa tctctagcct 9661 atcagctatt tgagtgggag aacctaccgc ctacgataaa ccctagtttc ttagaaaagt 9721 ctattcatca attcgggtac gtggggttct ataaagaccc tgtcatcagt tatatcgctt 9781 gtaatggcgc tctatcgggt cagagagacg tttacaacca agctacagtt tttagagccg 9841 catctcctgt gtatcaaaaa gaattcaagc tatacaacta tagagatatg aaggaagaag 9901 atatgggtgt tgttatctac aacaatgaca tggctttccc taccacgcca acgctagaat 9961 tgtttgcggc tgaattggct gaattaaaag aaatcatatc ggtcaaccaa aacgctcaaa 10021 agacacccgt cttaattaga gcaaatgaca ataaccaact gagcttaaaa caagtgtata 10081 accagtatga aggtaatgcc cctgttatct tcgctcacga agctctcgac agtgactcta 10141 tagaagtgtt taagactgat gctccctatg tggtggacaa gttaaacgct cagaaaaatg 10201 cagtatggaa tgagatgatg actttccttg gcattaagaa cgctaaccta gagaagaaag 10261 agcgcatggt tacggatgaa gtttccagta acgatgaaca gatcgagtct agcggcactg 10321 tatttttgaa gtcgagggaa gaagcatgtg agaagattaa tgagctatat ggtctcaatg 10381 ttaaagttaa attcagatat gacatcgtgg aacaaatgag acgtgagcta cagcaaatag 10441 aaaatgtttc acgtggaaca tcggacggtg aaacaaatga gtagttacac aatgcagtta 10501 agaacctaca ttgaaatgtg gtcacagggt gagacgggtt tatcaaccgc tgagaagata 10561 gagaaaggca gacccaagct attcgacttt aactatccta tctttgacga gtcataccgt 10621 accattttcg agacacattt cattcgcaat ttctatatgc gggagatcgg ttttgaaaca 10681 gaaggtttgt tcaagtttca tttagaaaca tggctcatga ttaatatgcc ctactttaac 10741 aagctttttg aaagtgagtt aatcaaatat gacccacttg aaaatactcg tgttggtgtg 10801 aagagtaaca cgaagaatga cacagataga aatgataacc gagacgttaa acaagattta 10861 acatcgaacg gaacatcatc tactgacgcc aaacagaacg acacgagtaa gacaacaggg 10921 aacgaaaaaa gtagcggtag cggctctata acagatgaca atttcaaacg tgatctaaat 10981 gcggataccg ctgatgaccg tttacagtta acaacaaaag acggtgaagg tgtcctagag 11041 tacgcttctc aaattgaaga acataacgag aataagaagc gagatacaaa gacaagtaat 11101 acaactgaca caactagcaa cacaacggga acaagtacac ttgattcgga ttccaagaca 11161 tccaacaaag cgaatacaac gagtaacgac aagttaaaca gtcagatcaa tagcgttgaa 11221 gattacatcg aagatagagt gggtaaaatc gggactcaaa gttatgctcg attggttatg 11281 gattaccgag aagctctact acgtatcgaa caaagaatct ttaacgaaat gcaagaactg 11341 tttatgcttg tgtattaagg aggacaacac atgtcaacta aacccgaatt gaaacggttt 11401 gagcagtttg gagagatgat ggttcagtta tatgaacggt atctaccaac cgcctttgat 11461 gaaagtttaa cattattgga gaaaatgaac aagatcatcc attacttgaa tgagattggc 11521 aaagtaacaa atgagctaat cgaagaatgg aacaaggtaa tggagtggat tctcaatgac 11581 gggttagaag acttagtcaa agagacgctc gaaagatggt atgaagaagg taaattcgct 11641 gacctcgtta tccaagttat cgacgaattg aagcagtttg gtgttagcgt taagacctat 11701 ggagcaaagg gcgacggtgt aactgatgat attagagctt ttgaaaaggc tatagagtct 11761 ggttttcctg tgtatgtgcc atatggaaca ttcatggttt cacgtggaat caaactacct 11821 agtaacaccg tattaaccgg ggcagggaaa cgtaatgctg tcattaaatt catggatagt 11881 gtgggacgtg gagaaagtct catgtataac gaaaatgtga caactggtaa cgaaaatatt 11941 ttcctgtctt cattcaccct cgatggtaat aataagcgct tgggtcaggg tatttcaggt 12001 atcggcggtt cacgggaaag taacctttct atccgtgcct gtcataacgt gtatatccgt 12061 gatattgaag ctgttgactg tacactacat ggaatcgaca tcacatgcgg tggactcgat 12121 tacccttact tgggtgatgg tacaactgct ccgaatccat cagaaaacat ctggattgaa 12181 aattgtgagg cgactggttt cggtgatgat gggatcacaa ctcaccacag ccaatacatc 12241 aatatcttga attgttactc acatgaccca cgtttaacag ctaactgtaa cgggtttgag 12301 attgatgacg gctcacgtca cgtggtgtta agtaataacc gttctaaagg ctgttacggt 12361 ggtatcgaga ttaaagctca cggagacgct ccggcggctt ataacatctc tataaacggt 12421 catatgtctg tagaagacgt tcggtcatat aacttccgac acatcggtca ccatgcggct 12481 actgccccgc agtccgtatc agctaaaaac attgttgctt cgaatcttgt atctattaga 12541 ccgaacaaca aacgtggttt ccaagacaat gctacgccga gggttctagc tgtatcagca 12601 tactacgggg ttgtaatcaa cggtttaaca gggtacacag atgaccctaa cctattgact 12661 gaaaccgttg tgtctgttca atttagggct agaaactgtt ctcttaatgg tgttgttctt 12721 actggattct ctaactctga gaatggaatc tatgtcattg gcggtagccg tggcggagac 12781 gccgttaata tcagtaacgt tacccttaat aactcaggta gatatggggt gtcaattggc 12841 tcaggaattg aaaacgtatc tatcaccaat attagcggta tcggtgacgg tattaattct 12901 cctgtagcac tcgtgtcaac gattaacagt aaccctgaga ttagcggatt aagtagtatt 12961 ggatacccta cggttgctag agttgcaggg acagactata acgatggttt aacactcttt 13021 aatggtgcgt ttcgtgcttc cacaacatct agcggcaaga ttcacagtga gggcttcatc 13081 atgggttcaa cgtcagggtg tgaagcatct gtttcaaaat caggtgttct aacgtcatca 13141 agctcgaaga ctagctctga gcgttctctc atagcaggta gttcaacatc tgaagccaag 13201 ggaacatata acacgatttt aggttcactg ggtgcggttg ctgatgagaa gtttgcggct 13261 ttaatttctg cgtcacaatc aagagcatca ggaaaccaca acttaattct atcaagttat 13321 ggaattaata caacagggtc atacaaagtt aacggtggat tcgaaaagat caactgggag 13381 ctagattcct taaacggtag aattaaagct cgtgacaccg taacaggcgg aaacacatgg 13441 agtgactttg ccgagtattt tgagagctta gacggtcaag tcattgaaac agggtatctg 13501 gttactcttg aaaaaggtaa aatccgtaaa gctgaaaaag gtgaaaagat catcggtgtt 13561 atcagtgaaa cagcggggtt tgtccttggt gaatcatcat ttgaatggca gggagctgtc 13621 cttaaaaacg agtttggcgg catcatttat gaggaagtca caacagaaga cggcgtaaaa 13681 ttcaaacgac cgcttccaaa ccctgacttt gaccctaata agaattatat cccacgtagt 13741 caacgtagag aatggcatgt tgttggttta ctggggcaga tcgctgttag aatcgacgaa 13801 acagttaaac aagggcatgg tattgatgct gttggcggtg ttgctacaga cggcgataac 13861 tttattgtgc aagaaataac taccccgtat acaaaggaaa aaggatacgg tgtggcgatc 13921 gtgttagtta agtgaggtag tctcctatgg tgtatgtatc taataaatat ctgacaatgt 13981 ctgagatgaa ggtcaacgct cagtacattc taaactacct cagtagtaac ggttggacaa 14041 agcaagccat ttgcgggatg ctcggtaata tgcaaagtga gagcaccata aatccgggat 14101 tatggcagaa tcttgacgag ggtaacacct cgttaggatt tgggcttgtg caatggacac 14161 ccgctagtaa ttatatcaat tgggctaaca gtcaaggact accatataag aacatggata 14221 gtgaattaaa gcgaattata tgggaagtaa acaataatgc tcaatggata aacttacgtg 14281 acatgacctt taaagagtac attaagagca cgaagacgcc tagagaacta gcgatgatat 14341 ttctagcatc ttatgagcga cctgctaacc ccaaccaacc cgaacggggc gaccaagccg 14401 aatactggta taaaaacctc agcggcggcg gaggcggtgg actacaatta gcacaattcc 14461 caatggatat tatcaatata tcgcaagggg aaaacggtag tttctcgcat aagggaacac 14521 tttgtataga ctttgtgggc aaaactgaaa agtacccgta ttatgctccg tgtgattgca 14581 catgtgtatg gcggggtgat gcgagtgcat atctagcgtg gacttctgat aaagaggtta 14641 tgtgtgcgga cggaagtgtt cgctatatta catgggtaaa cgttcatgaa agtcctttgc 14701 cctttgatgt tggtaagaag ctcaaaaaag gcgatctgat gggacacaca ggtatcggag 14761 gaaacgtaac aggcgaccat tggcacttca atgttattga cggtaaggag taccaaggat 14821 ggacaaagaa acctgattcg tgtttagcag ggacagagtt acacatatat gatgttttcg 14881 ctgtcaacaa cgtggagata atcaacggaa acggctacga ctggaaaact agtgattggc 14941 aagacggaga cggtggagat ggcgacgaca acgataacaa taaaacaaaa gatttaataa 15001 cccttttact atctgacgcc ctccatggtt ggaaagcata gaaaaggaga atgggagata 15061 tgaaaatgat agcgtggatg caacactttt tagagacaga cgaaacaaag cttatttact 15121 ggttaacatt ccttatggtt tgtatggttg ttgatacagt tttaggggtg ttatttgcaa 15181 agcttaaccc aaacattaaa ttttcatcat ttaaaatcaa aacaggggtg ttgattaaag 15241 tcagtgaaat gattctagcg ttattggcta tccctttcgc tgttcctttc cctgcgggtt 15301 tacccctatt atacacggtt tatacggctt tgtgtgtatc ggaaatatat tctattttcg 15361 gacatctgag attagtagat gataaaagtg attttcttga aatacttgaa aacttcttta 15421 agcgcacatc cggtaaaaat aaggaggaaa aataacatgc aaatttcaca agcgggtatc 15481 aacttaatta agagctttga gggtttacaa ctgaaagcat ataaagctgt tccgactgag 15541 aagcattaca ccattggtta cggtcattac ggttccgatg tttcacctag gcaggttatc 15601 actgctaaac aggctgaaga catgttgcgt gatgatgtgc aggcttttgt ggatggtgta 15661 aataaagcat taaaagtatc tgtcacccaa aatcaatttg atgcacttgt ctcattcgct 15721 tacaacgttg ggttaggggc tttcaggtct tcttctctac tggaatactt gaatgaagga 15781 agaacagctc tagcggcggc tgaattccct aaatggaata agtcaggcgg taaagtttat 15841 caagggttga ttaaccgtag agcacaggag caagccttgt ttaatagtgg aacacctaaa 15901 aatgtttcac gtggaacatc gtctactaaa acgacaccta agtataaggt gaagagtggt 15961 gacaacctta ctaaaatcgc taaaaagcat aatacaacgg ttgctacttt gttgaagttg 16021 aatccgagta tcaaagaccc gaacatgatt agagttggac aaacaataaa tgttacaggt 16081 agcggcggca aaacacataa ggtgaaaagt ggtgacacac tcagtaaaat tgccgttgat 16141 aacaaaacga ctgtgagtag attgatgagt ctaaaccctg aaattacgaa tccaaatcat 16201 ataaaagtag gtcaaacaat tagattaagt tgaggtgtaa atcatggaca agagtttatt 16261 ttataatcca cagaaaatgt tatcatacga tcgcatactg aactttgtta tcggtgctcg 16321 tggtatcggt aaatcatatg caatgaaggt gtaccctatt aatcgcttta ttaagtacgg 16381 agaacaattc atatatgttc gacgatacaa accggagctt gcgaaggtct ccaactattt 16441 taatgatgta gctcaagaat tccctgacca tgagttggtt gtgaagggta gaaggttcta 16501 cattgatggt aagcttgcag ggtgggctat tcctctgagt gtgtggcaga gtgaaaaatc 16561 taatgcatat cctaacgtaa gcacaatagt atttgatgag tttatcaggg agaaagacaa 16621 tagcaactat attcctaatg aggtttcagc tttactaaac cttatggaca ccgtatttcg 16681 taaccgtgag cgtgtcagat gcatttgttt aagtaatgct gtatccgttg ttaaccctta 16741 ttttctgttc ttcaaccttg tccctgatgt caacaaacgc ttcaatgtat atgacgatgc 16801 tttaattgaa atacctgata gtcttgactt ctcatctgaa aggcgtaaaa caaggtttgg 16861 gcggctaatt gatggaaccg agtacggtga gatgagttta gataaccagt ttatcggaga 16921 tagtcaggtg tttatagaaa agcgcagtaa ggatagtaag tttgtattct ccatcgtcta 16981 taatggattc actcttggtg tgtgggttga tgttaatcaa ggtcttatgt acattgatac 17041 agcacatgac ccgtcaacta agaatgtata cacattgaca acagatgatc ttaatgaaaa 17101 catgatgttg ataaccaact ataagaataa ttatcattta cgtaagttag ctagtgcgtt 17161 catgaatggt tatctgaggt ttgacaatca ggttatcaga aatatcgcat atgagttgtt 17221 tcgtaagatg cgtatacagt aatgtttcac gtggaacaat caaaagaaaa gcctatcgtc 17281 tgaggaacgg taggctcttt tgtagcatat agttgtattc atggaattgt ataatagtgt 17341 gtgacagcat ccagtctaaa acggcttgga attctttctg attcatctga tttcctcctc 17401 gatatgtgat actgaaagag attgatttcc acaggtggga cagaagacaa caggagtatc 17461 tttcttggtt attgcaccaa acacatcaaa ctttcgagtg aagaatgaga agctacagta 17521 gtgacaaacg tgtgtaagca acttcatttc aatgaccccc gatatagttt tttctgattc 17581 tccaatttcc agtatgtccg ctgtgtctct atatagttta acacttcctg ttcactttgt 17641 aaacgttgat taaccaaatc ttcgatgatg tcactaggaa tgcggatatt tgactgctca 17701 tacagatcaa gcacggcgac ttcacaagct gacaggttga ctgttttgtc aatgcttctg 17761 tttgagataa cttcgttaag ctcgctgagt atttgtgttc gttgaacaac tcgtttgttg 17821 aggtcttcaa gataatcttc tagctccctt gcttcctgtt tctttttgtt gtttcgtcct 17881 gatagaagga atataacgca caatgcaagt acaccgatca ttaaaatagc ttccatgtta 17941 tctccttgtc ccctttttat tggcgtgttg ccattctgaa ataactatat aacccatttc 18001 cctcatttta cggtcgatgt cttttaaaat atccacatga tgtatgtact cactaagtat 18061 catgttatcc atgtctacta gcatccatct gatgttagcc acaccaattg cataaatgtt 18121 gtttcttagt cgtgcacttg aacgtttcag tatcttgtga ccgtctgaga aagacaaagt 18181 gtaaaatact tcgtctccct ctggtgtttg aacagtgatt agccgcttgt cctcttttac 18241 aatgatgtct atcataatgt gaaattcatc ccctcaacga ctttatcaag attcttgatg 18301 tcttctgctg tttcctcatc aattggttca aatggtgtga tgtttgtcca aaacacagag 18361 tttgagcctt ctagtaactg accgctccat gatgcaaagc gtcttatcat tttagtatca 18421 gataagaatt gcgatgagta tgtagcaaag aacgttgggt tgtccttgag atagaaatgt 18481 aagatgcgtg tgccatttgt tgttagttgt tcgtagcata ccattcgcca acctcggcgc 18541 tctaagatga aagtgaatgc attaagttgt acgctcatgt tacttgttct ccttcttcat 18601 gctgtagata gatggtaagt ggttcttgaa tattttaagt tctgcttctt ctatagcttt 18661 gtcattaaag tcatgttctt gtattgactt ggcgatctta ttcgcttcat caagaagctc 18721 atcattgaat gtaacgattg acttatctac gtgtggtgat gtttcacgtg gaacatcaaa 18781 tctgataccg tctttatcaa tatagtcaac tctatctatt tcaccgtcta aatcttctaa 18841 ctcatcatct tcgtcgttgt cttgtgtgcc taactcttca acagttactt ctagtagcac 18901 tcttttaatg ctagatagtg caaagtcact gttaaggatg atagcgataa gttcttgttt 18961 gtctaagtcg tataagtctg taggaaatag attttctttc ttggctacaa gcttattgat 19021 ctctgtgttt gtgttgatga tgtcgattac ctcattgata gttaattgat agttattcat 19081 gtgtaattcc tcctatgatt ggttgtctta ttaccttact tctattatag tataacatgt 19141 taaacgatag tttgtctacc cttttcgaca aattgatgat aataaatagt ataggtatat 19201 agtcgtgatt tagttgttag attcttgtcg aagatagtcg gtcaatgggg aaatggtgta 19261 tgttgtcgct gtaccctact tt //